地龙糖浆纳米银水凝胶贴剂的制备及药效研究

Preparation and Pharmacodynamics Study of Hydrogel Patch Combining Earthworm Syrup with Nano-silver

关键词:  地龙糖浆  水凝胶贴剂  基质处方  响应面法  制备工艺

Key words:  earthworm syrup  hydrogel patch  matrix formulation  response surface methodology  preparation technology

摘要:

目的 研究地龙糖浆纳米银水凝胶贴剂的处方和制备工艺，并考察其对Ⅱ度烫伤的作用效果。方法 以蛋白质含量为指标，优选地龙糖浆制备中地龙和白糖的最佳比例。以初黏力、持黏力、剥离力及外观为指标，综合评价处方和成型工艺，单因素试验考察甘油、甘羟铝、聚丙烯酸钠的最佳用量，响应面法优化地龙糖浆纳米银水凝胶贴剂基质的最佳处方。构建小鼠Ⅱ度烫伤模型，以京万红烫伤膏为对照，通过小鼠创面愈合率、小鼠创面含水量、创面组织羟脯氨酸含量为指标，评价其药效。结果 地龙与白糖的最佳配比为1：0.8；最佳基质处方为甘油25%、甘羟铝0.4%、聚丙烯酸钠3.5%；该药物可明显提高小鼠创面组织愈合率，缩短愈合时间，缓解小鼠烫伤早期的水肿，增加创面羟脯氨酸的含量。结论 根据最佳处方所得的贴剂外观整洁，质地均匀，黏附性和剥离力符合中国药典2015年版的规定，对小鼠Ⅱ度烫伤有较好的疗效。

Abstract:

OBJECTIVE To study the prescription and preparation process of earthworm syrup nano-silver hydrogel patch, and evaluate the effect of promoting wound healing of deep second degree burn. METHODS Optimizing the ratio of the dragon and caster sugar in the preparation of hydrogel patch, taking the protein content as an indicator. The formulation process of hydrogel patch combining earthworm syrup with nano-silver was optimized by single factor experiment, which factors containing glycerol, glyceryl aluminum hydroxy, and sodium polyacrylate, then response surface methodology was used for evaluation of the formulation process, with stick power, shear strength, peel force and appearance as comprehensive indexes. The model of second degree scald in mice was constructed. The efficacy of the hydrogel patch was evaluated by wound healing rate, the water content and hydroxyproline content in the wound skin while the Jingwanhong scalding cream was used as control. RESULTS The fittest ratio between the earthworm and caster sugar was 1:0.8. The optimal formulation process of hydrogel patch was as follows:25% of glycerol, 0.4% of sodium glycerol, and 3.5% of sodium polyacrylate. The healing times of mice in the burn ointment groups were shorten. Meanwhile, the healing rates and the content of hydroxyproline were increased in the burn ointment groups. These edema in the early time of scald in mice were relieved. CONCLUSION The hydrogel patch prepared by the optimal formulation process has neat appearance with even quality and the adhesiveness meets the regulations of Chinese Pharmacopoeia 2015 Editon, and has a healing effect on deep second degree scald.