槲皮素通过PGC-1α通路对创伤性脑损伤的脑保护作用

Protective Effect of Quercetin on Traumatic Brain Injury Through PGC-1α Signal Pathway

关键词:  槲皮素  PGC-1α  创伤性脑损伤  神经细胞

Key words:  quercetin  PGC-1α  traumatic brain injury  nerve cell

摘要:

目的 研究槲皮素通过PGC-1α通路对创伤性脑损伤（traumatic brain injury，TBI）后神经细胞凋亡的影响。方法 选取48只ICR小鼠，随机分为4组，每组12只：假手术组，TBI组，TBI+溶剂组和TBI+槲皮素组。建立小鼠自由落体撞击脑损伤模型，TBI后30 min给予腹腔注射槲皮素。TBI 24 h后检测小鼠神经学功能评分和脑水肿情况；采用尼氏染色检测TBI后神经细胞凋亡情况； Western blotting检测小鼠神经细胞内外的PGC-1α及Bax、Bcl-2等凋亡相关蛋白的表达情况；采用免疫组化法进一步验证TBI后神经细胞PGC-1α的表达情况。结果 与假手术组相比，TBI组脑水肿与神经细胞凋亡明显增加（P < 0.01）； PGC-1α与Bax表达明显增加，而Bcl-2表达明显减少（P < 0.01）。与TBI+溶剂组相比，TBI+槲皮素组脑水肿及神经凋亡明显减轻（P < 0.05），Bax表达明显减少，PGC-1α和Bcl-2表达明显增加（P < 0.05）。结论 槲皮素可通过激活PGC-1α途径减轻TBI后神经细胞凋亡，起到有效的脑保护作用。

Abstract:

OBJECTIVE To explore the effect of quercetin on nerve cell apoptosis after traumatic brain injury(TBI) via PGC-1α signal pathway. METHODS Forty eight ICR mice were selected and randomly divided into four groups(n=12 in each group):sham operation group, TBI group, TBI+vehicle group and TBI+quercetin group. Mice model of free falling body impingement brain injury was established and quercetin was injected intraperitoneally 30 min after TBI. Brain edema and neurobehavioral assessments were detected 24 h after TBI. Nissl staining was used for obtain nerve cell apoptosis. The expression of PGC-1α, the level of Bax and Bcl-2 were detected by Western blotting analysis and immumohistochemical staining was used to verify the expression of nerve cell PGC-1α after TBI. RESULTS Compared with sham operation group, brain edema and nerve cell apoptosis of TBI group were increased significantly(P < 0.01). Expression of PGC-1α and Bax were increased significantly, and expression of Bcl-2 decreased(P < 0.01). Compared with TBI + vehicle group, brain edema and nerve cell apoptosis of TBI + quercetin group were decreased significantly(P < 0.05), expression of Bax were decreased significantly, and PGC-1α and Bcl-2 increased significantly(P < 0.05). CONCLUSION Quercetin can reduce the apoptosis of nerve cells after TBI by activating PGC-1α pathway and play an effective role in brain protection.