五味子乙素对2型糖尿病大鼠氧化应激和炎症反应的影响

Effects of Schisandrin B on Oxidative Stress and Inflammatory Response in Type 2 Diabetic Rats

关键词:  五味子乙素  2型糖尿病  糖脂代谢  氧化应激  炎症反应

Key words:  schisandrin B  type 2 diabetes  glycolipid metabolism  oxidative stress  inflammatory response

摘要:

目的 研究五味子乙素（schisandrin B，Sch B）对2型糖尿病大鼠氧化应激和炎症反应的影响。方法 采用腹腔注射链脲佐菌素及高糖高脂饮食构建2型糖尿病大鼠模型；给药组分别给予Sch B 40，80，120 mg·kg^-1灌胃；对照组和模型组给予2 mL·d^-1生理盐水灌胃，干预8周；称重法检测大鼠体质量变化，全自动化学分析仪检测FBG、TC、TG、HDL和LDL含量，HE染色观察肾损伤，Western blotting检测肾组织caspase-3、caspase-9、Bax、p-ACC、p-AMPK和p-LKB1表达，试剂盒检测血清MDA和SOD的水平，ELISA检测IL-6、IL-1β、IL-18的含量。结果 与模型组大鼠相比，给药组大鼠体质量明显增加，FBG、TC、TG和LDL含量明显减少，HDL含量明显增多，损伤程度明显减轻，caspase-3、caspase-9和Bax的表达明显下调，MDA含量明显减少、SOD活性明显增强，IL-6、IL-1b和IL-18的含量明显减少，p-ACC、p-AMPK和p-LKB1表达明显上调（P均<0.01）。结论 Sch B能够改善2型糖尿病大鼠体质量、糖脂代谢、肾损伤、氧化应激和炎症反应，并激活糖尿病大鼠的AMPK-LKB1信号转导通路。

Abstract:

OBJECTIVE To investigate the effect of schisandrin B(Sch B) on oxidative stress and inflammatory response in type 2 diabetic rats. METHODS A rat model of type 2 diabetes was established by intraperitoneal injection of streptozotocin and a high-sugar and high-fat diet. The administration group was given Sch B 40, 80, 120 mg·kg^-1, and the control and model groups were given 2 mL·d^-1 normal saline for 8 weeks. The weight changes of rats were detected by weighing method. The contents of FBG, TC, TG, HDL and LDL were detected by automatic chemical analyzer, and renal damage was observed by HE staining. The expressions of caspase-3, caspase-9, Bax, p-ACC, p-AMPK and p-LKB1 in renal tissues were detected by Western blotting. The levels of serum MDA and SOD were detected by kit. The content of IL-6, IL-1β and IL-18 were detected by ELISA. RESULTS Compared with the rats in the model group, the body weight of the rats in the administration group increased significantly, and the contents of FBG, TC, TG and LDL decreased significantly. The HDL content increased significantly, the degree of damage was significantly reduced, and the expression of caspase-3, caspase-9 and Bax was significantly down-regulated. The content of MDA was significantly decreased, the activity of SOD was significantly enhanced, the contents of IL-6, IL-1b and IL-18 were significantly decreased, and the expressions of p-ACC, p-AMPK and p-LKB1 were significantly up-regulated(all P<0.01). CONCLUSION Sch B can improve body weight, glycolipid metabolism, kidney injury, oxidative stress and inflammatory response in type 2 diabetic rats, and activate AMPK-LKB1 signal transduction pathway in diabetic rats.