甘草浸膏HPLC指纹图谱及多指标成分含量测定研究

Study on Fingerprint of Licorice Extract and the Content Determination of Its Multi-indicator

关键词:  甘草浸膏  高效液相色谱法  指纹图谱  多指标成分含量测定  甘草酸  黄酮类成分

Key words:  licorice extract  HPLC  fingerprint  determination of multi-indicator content  glycyrrhizic acid  flavonoids

摘要:

目的 建立甘草浸膏HPLC指纹图谱及多指标成分含量测定方法。方法 采用HPLC建立甘草浸膏的指纹图谱，并通过中药色谱指纹图谱相似度评价系统对13家企业的35批次样品进行相似度评价；以甘草苷、芹糖异甘草苷、异甘草苷、新异甘草苷、甘草查尔酮B和甘草酸6种成分为指标，采用Boston Green ODS色谱柱（4.6 mm×250 mm，5 μm），以乙腈（A）-0.1%甲酸（B）为流动相，梯度洗脱，建立了甘草浸膏的多指标HPLC含量测定方法。结果 建立了甘草浸膏HPLC指纹图谱，标定了22个共有峰，并指认了其中10个共有峰，35批样品相似度均>0.96，说明各批次甘草浸膏有较好的一致性；建立了6种指标成分含量测定方法，方法学考察符合规定。结论 本实验所建立的方法准确、简便、重复性好，可用于全面评价甘草浸膏的质量。

Abstract:

OBJECTIVE To establish an HPLC fingerprint of licorice extract and a method for determination of multi-index components. METHODS The fingerprint of licorice extract was established by HPLC, and the similarity evaluation of HPLC fingerprint of 35 batches of licorice extract from 13 manufacturers was carried out by the chromatographic fingerprint similarity evaluation system of traditional Chinese medicine. Six index components including glycyrrhizin, celery glycyrrhizin, isoglycyrrhizin, neoisoglycoside, licorice chalcone B and glycyrrhizic acid were used as indicators, and the multi-indicator HPLC method for the determination of licorice extract was established by using a Boston Green ODS (4.6 mm×250 mm, 5 μm) with acetonitrile(A)-0.1% formic acid(B) as the mobile phase gradient. RESULTS HPLC fingerprints of licorice extract were established, 22 common peaks were calibrated, and 10 common peaks were identified. The similarity of 35 batches of samples was >0.96, indicating that the different batches of licorice extract had good consistency. Six multi-indicator contents determination methods were established and methodological investigations were in compliance with the regulations. CONCLUSION The established methods are accurate, simple and reproducible, which can be used to comprehensively evaluate the quality of licorice extract.