云南松松塔醇提物对博来霉素诱导的肺纤维化小鼠的作用

Effect of Alcohol Extract from Pinecone of Pinus Yunnanensis Franch. on Bleomycin-induced Pulmonary Fibrosis in Mice

关键词:  云南松松塔  肺纤维化  博来霉素  信号传导与转录激活因子3

Key words:  Pinus yunnanensis Franch.  pulmonary fibrosis  bleomycin  STAT3

摘要:

目的 考察云南松松塔醇提物对肺纤维化模型小鼠的改善作用。方法 采用气管内一次性注射博来霉素制备肺纤维化模型，造模后第2天开始给予相应药物。实验第28天，眼球取血测定血细胞含量；取胸腺、脾脏和肺，计算免疫器官指数和肺脏指数；肺组织HE染色和Masson染色评价小鼠肺泡炎和肺纤维化情况，免疫组化测定肺组织中STAT3蛋白表达，ELISA法测定血清中IFN-γ、TNF-α、HYP水平和肺组织匀浆中TGF-β含量。结果 与模型组比较，松塔高剂量组小鼠血清TNF-α含量和肺组织匀浆TGF-β含量明显降低（P<0.05或P<0.01），上皮细胞中STAT3蛋白阳性表达颗粒明显减少，平均光密度降低（P<0.01）。松塔高、中剂量组小鼠血清中IFN-γ含量明显提高（P<0.01或P<0.05），松塔各剂量组HYP含量显著降低（P<0.01）。组织病理学结果显示，与模型组比较，松塔高剂量组肺泡炎及肺纤维化评分显著降低（P<0.01）。结论 云南松松塔醇提物对博来霉素诱导的肺纤维化小鼠具有改善作用，这种作用可能与减轻胶原沉积和减轻炎症反应有关。

Abstract:

OBJECTIVE To evaluate the amelioration of alcohol extracts from the pinecone of Pinus yunnanensis Franch. on pulmonary fibrosis in mice. METHODS The pulmonary fibrosis model was prepared by intratracheal injection of bleomycin. The corresponding drugs were given on the second day after modeling. On the 28th day of the experiment, the eyeball blood was taken to determine peripheral blood counts and the thymus, spleen and lung were harvested to calculate the immune organ index and lung index, respectively. Then the lung tissues were stained by HE and Masson to evaluate the pulmonary alveolitis and pulmonary fibrosis, respectively. The immunohistochemistry method was applied to determine the protein expression of STAT3. Finally the ELISA method was used to detect the serum levels of IFN-γ, TNF-α, HYP and the content of TGF-β in lung tissues homogenate. RESULTS Compared with model group, the contents of TNF-α in serum and TGF-β in lung tissues homogenate from Pinus yunnanensis Franch. high dose group decreased significantly(P<0.05 or P<0.01), the positive expression granule of STAT3 in lung tissue epithelial cells decreased significantly and the mean density descended (P<0.01). The serum IFN-γ content of the Pinus yunnanensis Franch. high and middle dose groups was significantly increased(P<0.01 or P<0.05), and the content of HYP in the Pinus yunnanensis Franch. groups was significantly decreased(P<0.01). Histopathological results showed that compared with model group, the pulmonary alveolar score and pulmonary fibrosis score in Pinus yunnanensis Franch. high dose group were decreased significantly(P<0.01). CONCLUSION The alcohol extract from pinecone of Pinus yunnanensis Franch. has an ameliorative effect on bleomycin-induced pulmonary fibrosis in mice, which may be related to reduction of collagen deposition and inflammation.