高灵敏共振瑞利散射技术快速检测药物中的美司那

Rapid Detection of Mesna in Drugs by Highly Sensitive Resonance Rayleigh Scattering Technique

关键词:  美司那  亮绿  共振瑞利散射

Key words:  mesna  bright green  resonance Rayleigh scattering

摘要:

目的 建立快速、准确测定药物中美司那的高灵敏共振瑞利散射（resonance Rayleigh scattering，RRS）新方法。方法 在BR弱碱性溶液中，亮绿与美司那以静电作用生成的缔合物使RRS信号显著增强，RRS强度在最大RRS峰处与美司那的浓度有线性关系。检测波长为344 nm。结果 在pH 7.75 BR缓冲溶液中，亮绿与美司那结合生成绿色二元离子缔合物，产生以294，344，468 nm为特征峰的新RRS光谱。最大RRS峰位于344 nm，线性范围为0.006~0.41 mg·L^-1，检出限为0.005 2 mg·L^-1。该法用于市售美司那药物中美司那的测定，加样回收率和相对标准偏差（RSD，n=6）分别为98.3%~103%和1.6%~2.3%。结论 该法简便、快速，有高灵敏度和高选择性，可用于实际药物中美司那的测定。

Abstract:

OBJECTIVE To establish a new high sensitivity resonance Rayleigh scattering(RRS) method for the rapid and accurate determination of mesna in drugs. METHODS In the weakly basic solution of BR, bright green was combined with mesna by electrostatic interaction to form ionic association complex, which led to a significantly enhanced of the RRS signal. The RRS intensity had a good linear relationship with mesna concentration at the peak of the maximum RRS. The detection wavelength was 344 nm. RESULTS In a BR buffer solution of pH 7.75, bright green combined with mesna to form a green binary ionic association. New RRS spectra with characteristic peaks at 294, 344, 468 nm was produced. The maximum RRS peak was located at 344 nm, linear range was 0.006-0.41 mg·L^-1, the detection limit was 0.005 2 mg·L^-1. This method had been applied to determine the content of mesna in commercially available mesna drugs, with the spiked recoveries and relative standard deviations(RSD, n=6) were 98.3% to 103% and 1.6% to 2.3%, respectively. CONCLUSION The method is simple, rapid. It has high sensitivity and selectivity, and it is suggested to be applied to the determination of mesna in the actual drug.