花生衣药材质量标准研究

Research on Quality Standards of Testa Arachis

关键词:  花生衣  薄层色谱法  高效液相色谱法  儿茶素

Key words:  Testa Arachis  TLC  HPLC  catechin

摘要:

目的 建立花生衣药材质量标准。方法 采用性状、显微鉴别法对该药材性状、显微特征进行描述；按照中国药典2015年版方法对水分、总灰分、酸不溶灰分进行测定；采用TLC定性鉴别；采用HPLC测定儿茶素的含量，Sepax BR-C₁₈色谱柱（4.6 mm×250 mm，5 μm），乙腈-水（10∶90）为流动相，流速1.0 mL×min^-1，柱温30℃，检测波长280 nm。结果 花生衣显微特征明显，专属性良好；TLC斑点清晰，分离度良好；水分、总灰分、酸不溶性灰分、浸出物、儿茶素的平均值分别为8.97%，6.0%，0.08%，23.7%，0.158%。含量测定儿茶素与相邻峰完全分离，线性关系良好，加样回收率（n=6）为106%，RSD为1.7%。结论 建立的方法可用于花生衣药材的质量控制。

Abstract:

OBJECTIVE To establish quality standards of Testa Arachis. METHODS Character and microscopic identification methods were adopted to observe morphological and histological characters. The moisture, total ash and acid-insoluble ash were determined according to the method of Chinese Pharmacopoeia 2015. TLC was used for qualitative identification. HPLC was used to determine the content of catechin, Sepax BR-C₁₈ column(4.6 mm×250 mm, 5 μm), acetonitrile-water(10:90) as mobile phase, flow rate was 1.0 mL·min^-1. The column temperature was 30℃, and the detection wavelength was 280 nm. RESULTS Testa Arachis had obvious microscopic characteristics and good specificity. TLC with clear spots and good separation. The average contents of water, total ash, acid insoluble ash content, extract and catechin constituent were 8.97%, 6.0%, 0.08%, 23.7% and 0.158%, respectively. In the assay, the catechin was completely separated from the adjacent peaks, and the linear relationship was good. The sample recovery rate(n=6) was 106%, and the RSD was 1.7%. CONCLUSION The established method can be used for the quality control of Testa Arachis.