霸王花不同部位粗多糖的结构组成及体外抗氧化活性

Structual Composition and in Vitro Antioxidant Activities of Crude Polysaccharide from Different Parts of Flower of Hylocereus Undatus

关键词:  霸王花  多糖  单糖组成  凝胶排阻色谱联用多角度激光光散射和示差折光仪法  红外光谱分析法

Key words:  flower of Hylocereus undatus  polysaccharide  monosaccharide compositions  SEC-MALLS-RID  FTIR

摘要:

目的 研究霸王花不同部位粗多糖的结构组成和体外抗氧化活性。方法 利用水提醇沉法制备霸王花4个不同部位（花瓣、花萼、雌蕊、雄蕊）的粗多糖，通过苯酚-硫酸法、考马斯亮蓝法、凝胶排阻色谱联用多角度激光光散射和示差折光仪法[size exclusion chromatography （SEC） coupled with multiangle laser light scattering （MALLS） and refractive index detector （RID），SEC-MALLS-RID]、高效液相色谱法、傅里叶红外光谱法（Fourier transform infrared spectrometer，FTIR）分析比较霸王花不同部位粗多糖结构特征；以对2，2-联氨-双（3-乙基苯并噻唑啉-6-磺酸）二胺盐自由基、羟基自由基和超氧阴离子自由基的清除作用为指标，评价霸王花不同部位粗多糖的体外抗氧化活性。结果 霸王花不同部位粗多糖中多糖含量为52.20%~61.52%，蛋白质含量为1.17%~3.21%；霸王花不同部位粗多糖均含有D-甘露糖、L-鼠李糖、D-葡萄糖醛酸、D-半乳糖醛酸、葡萄糖、D-半乳糖、D-木糖、D-阿拉伯糖，但单糖的比例有差异； 4个不同部位粗多糖的SEC色谱图、重均分子质量及其分布、FTIR色谱图具有相似性；霸王花不同部位粗多糖具有体外抗氧化活性。结论 霸王花不同部位粗多糖结构组成具有较高的相似性，均具有良好的抗氧化活性，在保健食品和药品等领域具有良好的开发利用前景。

Abstract:

OBJECTIVE To investigate the structual composition and in vitro antioxidant activity of crude polysaccharide from different parts of flower of Hylocereus undatus. METHODS The crude polysaccharide from petals, calyx, pistil and stamens in 4 different parts of flower of Hylocereus undatus were extracted by method of water extraction and alcohol precipitation. Structural characteristics of crude polysaccharide from different parts of flower of Hylocereus undatus were investigated and compared using phenol-sulfuric acid method, coomassie brilliant blue method, size exclusion chromatography(SEC) coupled with multiangle laser light scattering and (MALLS) refractive index detector(RID) (SEC-MALLS-RID), HPLC and Fourier transform infrared spectrometer(FTIR), respectively. The 2,2'-azinobis(3-ethylbenzthiazoline-6-sulphonate acid) radical, hydroxyl radical and superoxide anion radical scavenging activities was assayed to evaluate the antioxidant activity in vitro of crude polysaccharide from different parts of flower of Hylocereus undatus. RESULTS The results demonstrated that the contents of polysaccharides were 52.20%-61.52%, the protein contents were 1.17%-3.21% in crude polysaccharide of different parts from Hylocereus undatus. All polysaccharides contained D-mannose, L-rhamnose, D-glucuronic acid, D-galacononic acid, glucose, D-galactose, D-xylose and D-arabinose, but the proportion of monosaccharides varied remarkably in the crude polysaccharides from different parts of flower of Hylocereus undatus. The SEC chromatograms, the molecular weights and their distribution, and the FTIR chromatograms of crude polysaccharides from different parts of flower of Hylocereus undatus were similar. The crude polysaccharide from different parts of flower of Hylocereus undatus had significant anti-oxidative activities in vitro. CONCLUSION The structual composition of crude polysaccharide from different parts of flower of Hylocereus undatus have high similarity and significant anti-oxidative activities in vitro, and can be a resource of health foods and medicine for further development.