松木层孔菌净油化学成分分析与抗氧化和抗肿瘤活性评价

Chemical Composition Analysis of the Absolute Oil from Phellinus Pini and Its Antioxidant and Antitumor Activities Evaluation

关键词:  松木层孔菌  净油  化学成分  抗氧化  抗肿瘤

Key words:  Phellinus pini  absolute oil  chemical composition  antioxidant activity  antitumor activity

摘要:

目的 分析药用真菌松木层孔菌净油化学成分，评价其抗氧化和抗肿瘤活性。方法 用乙醚提取松木层孔菌净油，运用气相色谱串联质谱（GC-MS）分析净油化学成分，通过1，1-二苯基-2-苦基肼基（DPPH）清除试验评价其抗氧化活性，采用噻唑蓝（MTT）法评价其抗肿瘤活性。结果 鉴定了松木层孔菌净油中的11种成分，占净油总量的12.25%。生物活性试验结果表明，松木层孔菌净油有一定的抗氧化活性，清除DPPH自由基的IC₅₀值为0.82 mg·mL^-1，但对受试肿瘤细胞（Hela、A549和B16F10）抑制活性不明显，IC₅₀值均>100 μg·mL^-1。结论 松木层孔菌净油有一定的抗氧化活性，而且抗氧化活性随净油浓度的增大而增强，但抗肿瘤活性不明显。

Abstract:

OBJECTIVE To analysis the chemical composition of the absolute oil from the medicinal mushroom Phellinus pini, and to evaluate its antioxidant and antitumor activities. METHODS The absolute oil was isolated from Phellinus pini by ether, and its chemical composition were analyzed by GC-MS. The antioxidant activity was evaluated by 1,1-diphenyl-2- picrylhydrazino(DPPH) free radical scavenging test, and the antitumor activities were evaluated by MTT method. RESULTS Eleven components in the absolute oil from Phellinus pini were identified, accounting for 12.25% of the total amount of the absolute oil. The biological activity test showed that the absolute oil had a certain antioxidant activity, with the IC₅₀ value of 0.82 mg·mL^-1 against DPPH radical scavenging, but the inhibitory activities against the tested tumor cell lines (Hela, A549 and B16F10) were not obvious, all with the IC₅₀ values > 100 μg·mL^-1. CONCLUSION The absolute oil of Phellinus pini has a certain antioxidant activity, and the antioxidant activity increased with the increase of concentration of the absolute oil, but its antitumor activities are not obvious.