原位生物转化法提高槐角中染料木素的含量

Improvement of Genistein Content in Sophorae Fructus by in Situ Biotransformation

关键词:  染料木素  槐角苷  槐角  生物转化  米根霉

Key words:  genistein  sophoricoside  Sophorae Fructus  biotransformation  Rhizopus oryzae

摘要:

目的 开发一种生物转化工艺，将槐角中的槐角苷等糖苷转化为活性更高的染料木素，从而提高槐角的中药使用价值。方法 用米根霉（Rhizopus oryzae）LJH3菌株的粗酶液生物转化处理槐角，考察料液比、体系pH、槐角粒径大小和转化时间对提高槐角中染料木素含量的影响。结果 米根霉LJH3的粗酶液转化处理槐角后染料木素含量显著提高。在较优的转化条件下，即料液比5∶100，体系pH 5.0，槐角粒径0.2 mm，30℃条件下转化32 h，染料木素含量提高到36.3 mg·g^-1，较未转化时的含量提高了14.5倍。结论 利用米根霉LJH3的粗酶液原位生物转化槐角的转化工艺具有方法简单和产率高的优点，有较好的工业化应用价值。

Abstract:

OBJECTIVE To develop a biotransformation process to improve the medicinal value of the Chinese herb Sophorae Fructus by converting sophoricoside and other glycosides into genistein. METHODS Sophorae Fructus was treated by biotransformation using crude enzyme from Rhizopus oryzae LJH3. The effects of substrate-enzyme solution ratio, pH, particle size of substrate and conversion time on improving the content of genistein in Sophorae Fructus were investigated. RESULTS The content of genistein in Sophorae Fructus was significantly improved after it was treated with crude enzyme from Rhizopus oryzae LJH3. The optimum biotransformation conditions were as followings:substrate-enzyme solution ratio 5:100, pH 5.0, particle size of substrate 0.2 mm, temperature 30℃ and time 32 h. Under these conditions, the content of genistein in the biotransformed Sophorae Fructus increased to 36.3 mg·g^-1, which was 14.5 times higher than that in the untreated one. CONCLUSION The process by in situ biotransformation of Sophorae Fructus using crude enzyme from Rhizopus oryzae LJH3 has the advantages of simple operations and high productivity, which shows the potential application in the pharmaceutical industry.