HPLC同时测定半夏糖浆中琥珀酸、橙皮苷、甘草苷3种成分的含量

Simultaneous Determination of Succinic Acid, Hesperidin and Liquiritin in Banxia Syrup by HPLC

关键词:  HPLC  半夏糖浆  琥珀酸  橙皮苷  甘草苷

Key words:  HPLC  Banxia syrup  succinic acid  hesperidin  liquiritin

摘要:

目的 建立HPLC同时测定半夏糖浆中琥珀酸、橙皮苷、甘草苷3种成分的含量。方法 采用C₁₈色谱柱（4.6 mm×150 mm，5μm），以乙腈-0.2%磷酸溶液（18：82）为流动相，流速1.0 mL·min^-1，检测波长207 nm，柱温为常温。结果 3种待测成分分离度良好，阴性无干扰；3个成分线性范围分别为4.727~118.17 μg·mL^-1（r=0.999 9），2.474~61.857 μg·mL^-1（r=0.999 6），2.469~61.725 μg·mL^-1（r=0.999 9）；琥珀酸、橙皮苷、甘草苷平均回收率（n=9）分别为100.8%，99.3%，100.2%，RSD分别为1.3%，1.2%，1.8%，3批中琥珀酸、橙皮苷、甘草苷含量范围分别为0.072 2~0.079 4、0.029 9~0.034 8，0.022 8~0.029 0 mg·mL^-1。结论 该方法操作简单，缩短了分析时间，重复性好，可为半夏糖浆质量控制提供参考。

Abstract:

OBJECTIVE The high performance liquid chromatography(HPLC) method was established to simultaneously determine the level of succinic acid, hesperidin and liquiritin in Banxia syrup. METHODS The C₁₈ chromatographic column (4.6 mm×150 mm, 5 μm) was adopted, acetonitrile-0.2% phosphoric acid solution(18:82) was applied as mobile phase with flow rate of 1.0 mL·min^-1 under the wavelength of 207 nm. The column temperature was room temperature. RESULTS The compositions were separated perfectly without interference from negative controls. The liner range of Succinic acid, Hesperidin and liquiritin were 4.727-118.17 μg·mL^-1(r=0.999 9), 2.474-61.857 μg·mL^-1(r=0.999 6) and 2.469-61.725 μg·mL^-1(r=0.999 9), respectively. The average recovery rates of analytes(n=9) were 100.8%, 99.3% and 100.2% with their RSD value of 1.3%, 1.2% and 1.8%. The range level of succinic acid, hesperidin and liquiritin in Banxia syrup were 0.072 2-0.079 4 mg·mL^-1, 0.029 9-0.034 8 mg·mL^-1 and 0.022 8-0.029 0 mg·mL^-1. CONCLUSION It simplifies the composition analysis, reduces experiment time and ensures the repeatability, which may be used as an effective method for the quality control of Banxia syrup.