衢枳壳黄酮类成分调节胃肠动力的谱效关系研究

Study on Spectrum-activity Relationship of Flavonoids in Qu Aurantii Fructus for Regulation of Gastrointestinal Motility

关键词:  衢枳壳  调节胃肠动力  谱效关系

Key words:  Qu Aurantii Fructus  regulation of gastrointestinal motility  spectrum-activity relationship

摘要:

目的 研究衢枳壳甲醇提取物的HPLC指纹图谱及其调节胃肠动力的谱效关系。方法 采用小肠炭末推进模型和离体回肠收缩模型得到调节胃肠动力的药效结果，经LC-MS分析并指认衢枳壳甲醇提取物中的各色谱峰，运用多元回归分析(multiple regression analysis，MRA)和双变量相关分析(bivariate correlation analysis，BCA)，将衢枳壳甲醇提取物的药效数据与指纹图谱共有峰的相对峰面积相关联，分析谱效相关性。结果 采用LC-MS指认出12个色谱峰，经MRA和BCA，得出谱效方程(Y₁=52.379–0.255X₂+0.335X₃–4.499X₄+4.308X₅+0.750X₆，R²=0.959；Y₂=0.250+0.013 5X₂+0.023 0X₃+ 0.076 9X₆，R²=0.921)，其中5个是相关峰，2个色谱峰呈现极显著的相关性。采用不同样品代入谱效方程做拟合验证后，该拟合方程的预测值与实测值的拟合误差均<3%。结论 衢枳壳甲醇提取物中芸香柚皮苷(峰2)、柚皮苷(峰3)、柚皮素(峰4)、橙皮苷(峰5)、新橙皮苷(峰6)可能是衢枳壳中调节胃肠动力的效应物质，其中柚皮苷(峰3)和新橙皮苷(峰6)呈现极显著的相关性，与调节胃肠动力密切相关。

Abstract:

OBJECTIVE To study the spectrum-activity relationships between the HPLC fingerprints and the regulation of gastrointestinal motility activity of flavonoids in Qu Aurantii Fructus methanol extract. METHODS The models of intestinal carbon propulsion and contraction models of isolated ileum were established to obtaine the efficacy results of regulating gastrointestinal motility. The LC-MS was used to identify the components of Qu Aurantii Fructus methanol extract. Multivariate regression analysis(MRA) and bivariate correlation analysis(BCA) were used to associate efficacy data of Qu Aurantii Fructus methanol extract with the relative peak area of the fingerprint common peak to analyze the spectral effect correlation. RESULTS The 12 components were identified by LC-MS, studied by MRA and BCA, to obtain the spectrum efficiency equation (Y₁=52.379-0.255X₂+0.335X₃-4.499X₄+4.308X₅+0.750X₆, R²=0.959; Y₂=0.250+0.013 5X₂+0.023 0X₃+0.076 9X₆, R²=0.921). It was showed five peaks were the correlated peaks, and 2 of them were significantly correlated. After fitting verification for different samples substituted into the spectrum-activity equation, the fitting error between the predicted value and the measured value of the fitting equation was <3%. CONCLUSION Narirutin(peak 2), naringin(peak 3), naringenin(peak 4), hesperidin(peak 5) and neohesperidin(peak 6) in Qu Aurantii Fructus methanol extract may be the material foundation in Qu Aurantii Fructus methanol extract for regulation of gastrointestinal motility, in which naringin(peak 3) and neohesperidin(peak 6) are significant correlation.