HPLC波长转换法同时测定蒙药文冠木中5种成分的含量

Simultaneous Determination of Five Components in Mongolian Medicine Xanthoceras Sorbifolia Bunge by HPLC Wavelength Conversion Method

关键词:  文冠木  HPLC波长转换法  质量控制

Key words:  Xanthoceras sorbifolia Bunge  HPLC wavelength conversion method  quality control

摘要:

目的 建立同时测定蒙药文冠木药材中儿茶素、表儿茶素、槲皮苷、槲皮素和杨梅素5种成分含量的方法，为文冠木药材及制剂的质量控制提供参考和依据。方法 采用HPLC波长转换法，色谱柱为Agilent Eclipse Plus C₁₈柱（4.6 mm×250 mm，5 μm），以乙腈（A）-0.1%磷酸水溶液（B）为流动相进行梯度洗脱，流速为1.0 mL·min^-1；检测波长分别为0~8 min，280 nm（儿茶素，表儿茶素）、8~10 min，257 nm（槲皮苷）、10~12 min，375 nm（杨梅素）和12~18 min，256 nm（槲皮素）；柱温30℃；进样量为10 μL。结果 儿茶素、表儿茶素、槲皮苷、杨梅素及槲皮素分别在6.64~33.20 μg·mL^-1（r²=0.999 6）、49.04~245.20 μg·mL^-1（r²=0.999 7）、1.28~6.40 μg·mL^-1（r²=0.999 8）、14.4~72.0 μg·mL^-1（r²=0.999 2）、0.84~4.20 μg·mL^-1（r²=0.999 6）线性关系良好；仪器精密性、稳定性、重复性的RSD均<2.0%；平均加样回收率分别为98.15%（RSD=1.4%，n=6），102.34%（RSD=1.4%，n=6），91.90%（RSD=1.3%，n=6），101.16%（RSD=1.9%，n=6），94.97%（RSD=1.6%，n=6）。结论 该方法操作简单便捷，仪器精确度高，仪器精密度、稳定性、重复性好，可用于同时测定文冠木的质量控制。

Abstract:

OBJECTIVE To establish a method for simultaneous determination of catechin, epicatechin, quercitrin, quercetin, myricetin in Xanthoceras sorbifolia Bunge, and to provide reference and basis for the quality control of Xanthoceras sorbifolia Bunge and its preparation. METHODS HPLC wavelength conversion method was adopted. Agilent Eclipse Plus C₁₈ column(4.6 mm×250 mm, 5 μm)was used; the mobile phase was acetonitrile(A)-0.1% phosphoric acid solution(B) at a flow rate of 1.0 mL·min^-1; the detection wavelength was 0-8 min, 280 nm(catechin, epicatechin), 8-10 min, 257 nm(quercitrin), 10-12 min, 375 nm(myricetin) and 12-18 min, 256 nm(quercetin); the column temperature was 30 ℃; the injection volume was 10 μL. RESULTS The linear ranges of catechin, epicatechin, quercitrin, quercetin, myricetin were 6.64-33.20 μg·mL^-1(r²=0.999 6), 49.04-245.20 μg·mL^-1(r²=0.999 7), 1.28-6.40 μg·mL^-1(r²=0.999 8), 14.4-72.0 μg·mL^-1(r²=0.999 2), 0.84-4.20 μg·mL^-1(r²=0.999 6) respectively, the five components showed good linear correlations; RSD of precision, stability and repeatability were all< 2.0%; the average recoveries were 98.15%(RSD=1.4%, n=6), 102.34%(RSD=1.4%, n=6), 91.90%(RSD=1.3%, n=6), 101.16% (RSD=1.9%, n=6), 94.97%(RSD=1.6%, n=6). CONCLUSION The method is simple and convenient, with high precision, stability and repeatability, and can be used for quality control of Xanthoceras Sorbifolia Bunge.