基于ERK/p38 MAPK信号通路探讨复方玄参利咽合剂对慢性咽炎模型兔抗炎机制研究

Study on Anti-inflammatory Mechanism of Compound Xuanshen Liyan Mixture on Chronic Pharyngitis Model Rabbits Based on ERK/p38 MAPK Signaling Pathway

关键词:  复方玄参利咽合剂  慢性咽炎  彭氏造模法  白细胞介素-1β  肿瘤坏死因子-α  前列腺素E2  兔

Key words:  Compound Xuanshen Liyan mixture  chronic pharyngitis  Peng's modeling method  IL-1β  TNF-α  PGE2  rabbit

摘要:

目的 研究复方玄参利咽合剂减轻慢性咽炎模型兔炎症反应的作用机制。方法 利用彭氏造模法咽部喷2.5%氨水和咽部黏膜下注射0.5 mL松节油复合因素建立新西兰兔的慢性咽炎模型，随机分为模型对照组、金果饮组、复方玄参利咽合剂组，另取正常新西兰兔为正常对照组。观察复方玄参利咽合剂对慢性咽炎兔咽喉黏膜病理改变的影响，ELISA检测血清中白细胞介素-1β（IL-1β）、肿瘤坏死因子-α（TNF-α）和前列腺素E2（PGE2）含量的变化，Western blotting检测兔咽部组织中p38、P-p38、ERK1/2及P-ERK1/2的表达。结果 与正常对照组比，慢性咽炎模型对照组病理表现黏膜层不平整，部分细胞坏死、脱落，黏膜下层结构被破坏，血清中IL-1β、TNF-α和PGE2均极显著升高（P<0.01），咽部组织中P-p38与P-ERK1/2蛋白表达水平极显著上升（P<0.01），提示造模成功。与模型对照组相比，复方玄参利咽合剂组显示病理状态有所好转，血清中炎性指标IL-1β、TNF-α和PGE2均显著降低（P<0.01），咽部组织中P-p38与P-ERK1/2蛋白表达水平显著降低（P<0.01）。结论 复方玄参利咽合剂可能通过降低MAPK信号通路中P-p38与P-ERK1/2蛋白表达水平减轻慢性咽炎兔炎症反应。

Abstract:

OBJECTIVE To study the mechanism of Compound Xuanshen Liyan mixture in reducing the inflammatory response of chronic pharyngitis model rabbits. METHODS The chronic pharyngitis model of New Zealand rabbits was established by using the combined factors of 2.5% ammonia injection in pharynx and 0.5 mL turpentine injection in submucosa of pharynx by Peng's modeling method, which were divided into model control group, Jinguoyin group, Compound Xuanshen Liyan mixture group, normal New Zealand rabbits were taken as normal control group. Observed the effect of Compound Xuanshen Liyan mixture on the pathological changes of pharyngeal mucosa in rabbits with chronic pharyngitis, and detected the changes of serum IL-1β, TNF-α and PGE2 by ELISA, and detected the expressions of p38, P-p38, ERK1/2 and P-ERK1/2 in pharyngeal tissues by Western blotting. RESULTS Compared with the normal control group, in the model control group of chronic pharyngitis, the mucosa was not smooth, some cells died and fell off, and the submucosal structure was destroyed, IL-1β, TNF-α and PGE2 in serum were significantly increased(P<0.01), the expression levels of P-p38 and P-ERK1/2 proteins in pharyngeal tissues were significantly increased(P<0.01), indicated a successful modeling. Compared with the model control group, in the Compound Xuanshen Liyan mixture group, the pathological state was improved, the inflammatory indicators IL-1β, TNF-α and PGE2 in serum were all significantly decreased(P<0.01), and the protein expression levels of P-p38 and P-ERK1/2 in pharyngeal tissues were significantly decreased(P<0.01). CONCLUSION Compound Xuanshen Liyan mixture may reduce the inflammatory response of rabbits with chronic pharyngitis by reducing the expression levels of P-p38 and P-ERK1/2 proteins in the MAPK signaling pathway.