Box-Behnken响应面法优化高良姜挥发油的包合工艺研究

Optimization for Inclusion Process of Alpinia Officinarum Hance Volatile Oil by Box-Behnken Response Surface Methodology

关键词:  高良姜挥发油  β-环糊精  胶体磨  包合工艺  响应面优化

Key words:  Alpinia officinarum Hance volatile oil  β-cyclodextrin  colloid mill  inclusion process  response surface optimization

摘要:

目的 优化胶体磨法制备高良姜挥发油β-环糊精（β-cyclodextrin，β-CD）包合物的工艺。方法 在单因素试验基础上采用Box-Behnken响应面法，以包合物得率和包合率为评价指标，考察β-CD与高良姜挥发油的比例、水与β-CD的比例以及包合时间对包合工艺的影响；通过红外光谱法和显微影像法初步验证高良姜挥发油β-CD包合物的生成。结果 高良姜挥发油的最佳包合工艺为β-CD与高良姜挥发油的比例为10∶1，水与β-CD的比例为5∶1，包合时间为45 min。结论 胶体磨法包合高良姜挥发油合理、可行。

Abstract:

OBJECTIVE To optimize the preparation process of Alpinia officinarum Hance volatile oil wrapped with b-cyclodextrin(b-CD) using the colloid mill method. METHODS With yield of inclusion compound and inclusion rate as evaluation indexes, the single factor test combining Box-Behnken response surface methodology was used to study the effects of the ratio of b-CD to Alpinia officinarum Hance volatile oil, the ratio of water to b-CD, and inclusion time on the inclusion process. Inclusion complexes were verified by IR and microscopic image. RESULTS The optimized parameters of inclusion were as follows:the ratio of b-CD to Alpinia officinarum Hance volatile oil was 10:1, the ratio of water to b-CD was 5:1, and the inclusion time was 45 min. CONCLUSION The inclusion of Alpinia officinarum Hance volatile oil by colloid mill is reasonable and feasible.