| 引用本文: | 陈智,林圣云,李建新,李晖,熊昊,聂应明,裘玫.白花蛇舌草乙醇提取物体外诱导人AML-M2白血病细胞凋亡机制研究[J].中国现代应用药学,2020,37(17):2067-2072. |
| CHEN Zhi,LIN Shengyun,LI Jianxin,LI Hui,XIONG Hao,NIE Yingming,QIU Mei.Study on the Mechanism of Inducing Apoptosis of AML-M2 Cells in Vitro by Ethanol Extraction of Hedyotis Diffusa Willd.[J].Chin J Mod Appl Pharm(中国现代应用药学),2020,37(17):2067-2072. |
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| 白花蛇舌草乙醇提取物体外诱导人AML-M2白血病细胞凋亡机制研究 |
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陈智,林圣云,李建新,李晖,熊昊,聂应明,裘玫
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1.华中科技大学同济医学院附属武汉儿童医院血液肿瘤科, 武汉 430016;2.浙江中医药大学附属第一医院血液科, 杭州 310006;3.湖北中医药大学中医临床学院, 武汉 430061
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| 摘要: |
| 目的 观察白花蛇舌草乙醇提取物（ethanol extract of Hedyotis diffusa Willd.，EEHDW）对AML-M2白血病Kasumi-1细胞增殖和凋亡的影响机制。方法 采用MTT比色法、Hoechest荧光染色检测测定EEHDW作用后Kasumi-1细胞增殖和凋亡的情况，Western blotting法检测Kasumi-1细胞中Bcl-2、Bax、caspase-3、caspase-9、Cyto-C、P65、p-P65、IkBα、p-IkBα、IKKα/β、C-myc以及AML1-ETO的蛋白水平。结果 EEHDW抑制急性髓系白血病Kasumi-1细胞增殖，并具有时间和浓度依赖性。0.04，0.06，0.08 mg·mL-1的EEHDW能诱导细胞凋亡（P<0.05或P<0.01）。EEHDW可以显著上调Cyto-C、cleaved PARP、cleaved caspase-3、cleaved caspase-9以及Bax的表达水平，而降低Bcl-2、C-myc和AML1-ETO蛋白的表达，同时EEHDW能够浓度依赖性地增加p-P65、p-IkBα的蛋白表达。结论 EEHDW诱导Kasumi-1细胞凋亡一方面是通过调节Bax/Bcl-2的表达影响线粒体途径，另外一方面还与激活NF-кB信号通路有关，在这个过程中同时抑制原癌基因C-myc和AML1-ETO融合基因的表达。 |
| 关键词: 白花蛇舌草乙醇提取物 Kasumi-1细胞 凋亡 NF-kB信号通路 |
| DOI：10.13748/j.cnki.issn1007-7693.2020.17.004 |
| 分类号:R965.1 |
| 基金项目:湖北省卫生计生委面上项目（WJ2017M195）；武汉市中青年医学骨干人才培养计划（武卫生计生[2014]77号）；武汉市卫计委临床医学科研项目（WX13B19） |
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| Study on the Mechanism of Inducing Apoptosis of AML-M2 Cells in Vitro by Ethanol Extraction of Hedyotis Diffusa Willd. |
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CHEN Zhi1,2, LIN Shengyun3, LI Jianxin1,2, LI Hui1,2, XIONG Hao1,2, NIE Yingming1,2, QIU Mei4
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1.Department of Hematology, Wuhan Children's Hospital, Tongji Medical College, Huazhong University of Science &2.Technology, Wuhan 430016, China;3.Department of Hematology, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou 310006, China;4.Clinical College of Chinese Medicine, Hubei University of Chinese Medicine, Wuhan 430061, China
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| Abstract: |
| OBJECTIVE To observe the effect of ethanol extract of Hedyotis diffusa Willd. (EEHDW) on the proliferation and apoptosis of AML-M2 cells Kasumi-1 and its mechanism. METHODS MTT colorimetry and Hoechest staining were used to detect the effect of EEHDW on the proliferation and apoptosis of Kasumi-1 cells. Western blotting was used to detect the protein levels of Bcl-2, Bax, caspase-3, caspase-9, Cyto-C, p65, p-P65, IkBa, p-IkBa, IKKa/b, C-myc and AML1-ETO in Kasumi-1 cells. RESULTS EEHDW inhibited the proliferation of Kasumi-1 cells. The 0.04, 0.06 and 0.08 mg·mL-1 of EEHDW could induce apoptosis(P<0.05 or P<0.01). It could significantly up regulate the expression of Cyto-C, cleaved PARP, cleaved caspase-3, cleaved caspase-9 and Bax, but decreased the expression of Bcl-2, C-myc and AML1-ETO. Meanwhile, EEHDW could increase the expression of p-P65 and p-IKBα in a concentration dependent manner. CONCLUSION EEHDW induce apoptosis of Kasumi-1 cells not only related to the regulation of Bax/Bcl-2 expression, but also relate to the activation of NF-кB signal pathway, which inhibits the expression of C-myc and AML1-ETO fusion gene. |
| Key words: ethanol extract of Hedyotis diffusa Willd. Kasumi-1 cell apoptosis NF-kB signal pathway |
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