红芪总多糖对脑缺血再灌注损伤大鼠脑、心的保护作用

Protective Effect of Total Hedysari Radix Polysaccharide on Brain and Heart of Rats with Cerebral Ischemia-reperfusion Injury

关键词:  红芪总多糖  脑缺血再灌注损伤  脑  心  保护作用

Key words:  total Hedysari Radix polysaccharide  cerebral ischemia-reperfusion injury  brain  heart  protective effect

摘要:

目的 研究红芪总多糖对脑缺血再灌注损伤大鼠脑、心的保护作用。方法 线栓法建立大鼠脑缺血再灌注模型，观察红芪总多糖对大鼠神经功能学评分、脑指数、脑梗死体积百分比、脑含水量，心肌组织病理变化、心脏脏器指数、氧化与抗氧化代谢状况以及DNA结构的变化等指标的影响。结果 红芪总多糖（200 mg·kg^-1）能明显改善脑缺血再灌注损伤所引起的神经缺损症状、脑梗死体积百分比和脑含水量，同时降低心脏脏器指数、心肌梗死面积、心肌组织病理学病变和自由基损伤程度。结论 脑缺血再灌注不仅可以引起脑组织损伤，而且对远隔器官心脏也有不同程度的损害。而红芪总多糖对脑、心有明显保护作用，其作用机制可能与提高大鼠抗氧化损伤能力有关。

Abstract:

OBJECTIVE To study the protective effect of total Hedysari Radix polysaccharide on brain and heart of rats with cerebral ischemia-reperfusion injury. METHODS The model of cerebral ischemia-reperfusion injury was established by suture method in rats. The score of neurological deficit, cerebral index, percentage of cerebral infarction volume and brain water content were observed. Meanwhile, the changes of pathological tissue, heart organ index, oxidation and antioxidant metabolism and changes of DNA structure in myocardial tissue of rats were also detected. RESULTS Total Hedysari Radix polysaccharide(200 mg·kg^-1) could obviously improve the neurological deficit, percentage of cerebral infarction volume, the brain water content caused by cerebral ischemia-reperfusion injury. Heart organ index, the rate of heart infarction, the degree of myocardial pathological changes and the level of free radical damage could be also significantly decreased. CONCLUSION Cerebral ischemiareperfusion can not only damage brain tissue, but also hurt the heart in different degrees. Total Hedysari Radix polysaccharid has the protective effect on heart and brain of rats with cerebral ischemia-reperfusion injury which may be associated with the enhancement of antioxidant capability of rats.