| 引用本文: | 刘东彦,石晓峰,李冲,王东东,范彬,沈薇,马趣环.雪松松针中莽草酸的分离及其纯度检查[J].中国现代应用药学,2011,28(7):637-640. |
| LIU Dongyan,SHI Xiaofeng,LI Chong,WANG Dongdong,FAN Bin,SHEN Wei,MA Quhuan.Separation of Shikimic Acid from Pine Needles of Cedrus Deodara and Its Purity Determination[J].Chin J Mod Appl Pharm(中国现代应用药学),2011,28(7):637-640. |
|
| 摘要: |
| 目的 从雪松松针中提取分离莽草酸并进行纯度检查。方法 采用水提、萃取、柱层析、重结晶等步骤分离纯化莽草酸，用NMR和理化性质鉴定其结构，再采用TLC和HPLC对其纯度进行检查。结果 分离得到的莽草酸纯度达95%以上，得率为0.72%。结论 该法简便经济、合理可行，可用于雪松松针中莽草酸的提取分离。 |
| 关键词: 雪松松针 莽草酸 分离 纯度检查 |
| DOI： |
| 分类号: |
| 基金项目:甘肃省技术研究与专项计划项目(0709TCYA022)；甘肃省卫生厅科研计划项目(WST07-02) |
|
| Separation of Shikimic Acid from Pine Needles of Cedrus Deodara and Its Purity Determination |
|
LIU Dongyan1,2, SHI Xiaofeng3, LI Chong4, WANG Dongdong4, FAN Bin3, SHEN Wei3, MA Quhuan3
|
|
1. Gansu Academy of Medical Science, Lanzhou 730050, China;2. School of Pharmacy, Lanzhou University, Lanzhou 730030, China;3.Gansu Academy of Medical Science, Lanzhou 730050, China;4.School of Pharmacy, Lanzhou University, Lanzhou 730030, China
|
| Abstract: |
| OBJECTIVE To separate shikimic acid from pine needles of Cedrus deodara and determine its purity. METHODS Shikimic acid was separated by process of water extraction, solvent extraction, silica gel column chromatography, recrystallizing etc. Its structure was identified on the basis of NMR analysis and chemical evidence and its purity was determined by TLC and HPLC. RESULTS The purity of shikimic acid was more than 95% and the total yield was 0.72%. CONCLUSION The method is convenient and reasonable, it can be used to separate the shikimic acid from pine needles of Cedrus deodara. |
| Key words: pine needles of Cedrus deodara shikimic acid separate purity determination |
