| 引用本文: | 陈枢青;韩晓旭.利用电泳滴定曲线选择多核苷酸磷酸化酶快速离子交换层析的最佳条件[J].中国现代应用药学,1999,(1):34-36. |
| Chen Shuqing(Chen SQ),Han Xiaoxu(Han XX).Use of electrophoretic titration curve for predicting optimal chromatographic conditions for purification of polynucleotide phosphorylase by fast ion-exchange chromatography[J].Chin J Mod Appl Pharm(中国现代应用药学),1999,(1):34-36. |
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| 关键词: 电泳滴定曲线 多核苷酸磷酸化酶 快速离子交换层析 纯化 |
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| Use of electrophoretic titration curve for predicting optimal chromatographic conditions for purification of polynucleotide phosphorylase by fast ion-exchange chromatography |
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Chen Shuqing(Chen SQ),Han Xiaoxu(Han XX)
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| Abstract: |
| OBJECTIVE:Selection of optimal chromatographic conditions for purification of polynucleotide phosphorylase in fast ion-exchange chromatography.METHODS:Preparation of electrophoretic titration curve of E. coli extract and prediction of optimal conditions f |
| Key words: Electrophoretic titration curve,polynucleotide phosphorylase,fastion-exchange chromatography,puritication |
