| 引用本文: | 曾令高;范能全;曾杰;刘映倩.衍生化紫外分光光度法测定新霉素含量[J].中国现代应用药学,2006,(6):494-495. |
| ZENG Ling-gao,FAN Neng-quan,ZENG Jie,LIU Ying-qian.Determination of neomycin by derivatization with UV-spectrophotometry[J].Chin J Mod Appl Pharm(中国现代应用药学),2006,(6):494-495. |
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| 摘要: |
| 目的建立硫酸新霉素中新霉素的含量测定方法。方法以乙酰丙酮-甲醛为衍生化试剂的紫外分光光度法,检测波长为285nm。结果新霉素在2.69～14.34μg·mL-1浓度范围内吸收度与浓度呈良好线性关系,r=0.999 7,平均回收率为98.63%。结论该法准确,快速,结果满意。 |
| 关键词: 衍生化 紫外分光光度法 新霉素 含量 |
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| Determination of neomycin by derivatization with UV-spectrophotometry |
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ZENG Ling-gao,FAN Neng-quan,ZENG Jie,LIU Ying-qian
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| Abstract: |
| OBJECTIVE To establish the method for determination of neomycin.METHODS A UV-spectrophotometry method by derivatization was used.The derivative reagent was acetylacetonate-formaldehyde. RESULTS The wavelength of detection was 285nm.The calibration curve w |
| Key words: derivatization UV-spectrophotometry neomycin content |
