大孔树脂分离纯化延胡索总生物碱的工艺研究

Study on the Technology of Isolation and Purification of Gross Aalkaloid in Rhizoma Corydalis with Macropopous Adsorbing Resin

关键词:  大孔树脂  分离  纯化  延胡索  总生物碱

Key words:  macropopous adsorbing resin  isolation  purification  Rhizoma Corydalis  gross alkaloid

摘要:

目的研究大孔树脂分离纯化延胡索总生物碱的工艺条件及参数。方法以延胡索总生物碱提取率为评价指标筛选大孔树脂。结果经试验表明分离纯化的最佳工艺为D-101大孔树脂，上样量为延胡索药材与树脂体积比为1∶1（g·mL-1）；洗脱速度0.5 BV·h-1；去离子水洗脱杂质，洗脱体积为6 BV，80％乙醇洗脱总生物碱，洗脱体积为6 BV。结论该工艺简单可行，分离效果好，能满足于大生产的要求。

Abstract:

OBJECTIVE To explore the purification method of gross alkaloid in Rhizoma Corydalis with macropopous adsorbing resin.METHODS The extracting yield of the gross alkaloid were taken as index to select the macroporous resin.RESULTS Optium conditions for isolation and purification were indentified, I.e. D-101 type macropopous adsorbing resin,adsorbing rate was 0.5 BV·h-1,water volume for removing impurities was 6 BV,concentration of the ethanol was 80%,and volume of ethanol was 6 BV.CONCLUSION The method is simple and feasible,with good separating effect which can meet industrial requirements.