| 引用本文: | 王增,祝宇华,杨兵勋,陈立钻,楼正家,沈春香,杨芳亮,夏克中,贾婷婷.HPLC测定铁皮石斛饮料中人参皂苷Rb1的含量[J].中国现代应用药学,2010,27(13):1206-1208. |
| .Determination of the Content of Ginsenoside Rb1 in Tiepishihu Beverage by HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2010,27(13):1206-1208. |
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| 摘要: |
| 目的 建立测定铁皮石斛饮料中人参皂苷Rb1含量的方法。方法 采用Agilent Eclipse XDB-C18(4.6 mm×250 mm，5 µm)色谱柱；流动相：乙腈-0.1%磷酸溶液(32∶68)；流速：1.0 mL·min-1；进样体积：10 µL，柱温：40 ℃；检测波长：203 nm。结果 人参皂苷Rb1在1.012~5.06 µg内呈良好的线性关系(r=1.000 0)，平均加样回收率为102.3%，RSD为1.8%(n=6)。结论 该法准确，简便，快速，灵敏度高，重复性好，适用于铁皮石斛饮料中人参皂苷Rb1的含量测定。 |
| 关键词: 铁皮石斛 人参皂苷Rb1 高效液相色谱法 |
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| Determination of the Content of Ginsenoside Rb1 in Tiepishihu Beverage by HPLC |
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WANG Zeng ZHU Yuhua YANG Bingxun CHEN Lizuan LOU Zhengjia SHEN Chunxiang YANG Fangliang XIA Kezhong JIA Tingting
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| Abstract: |
| OBJECTIVE To establish an HPLC method for determination of the content of ginsenoside Rb1 in Tiepishihu beverage. METHODS An Agilent Eclipse XDB-C18 column(4.6 mm×250 mm, 5 µm) was used. The mobile phase consisted of acetonitrile-0.1% phosphoric acid solution(32∶68), the flow rate was 1.0 mL·min-1, the wavelength of detector was 203 nm, the injection volume was 10 µL and column temperature was set at 40 ℃. RESULTS The calibration curves showed good linearity in the range of 1.012-5.06 μg. The average recovery(n=6) was 102.3%, RSD was 1.8%. CONCLUSION The method was simple, fast and accurate for analysis of ginsenoside Rb1 in Tiepishihu beverage. |
| Key words: Tiepishihu beverage ginsenoside Rb1 HPLC |
