| 引用本文: | 黄冬芳,梁洁,韦金玉,祁金丽,曹玉嫔,梁晶春,安施佳,刘星晨,滕建北.荔枝叶药材质量标准研究[J].中国现代应用药学,2023,40(14):1967-1975. |
| HUANG Dongfang,LIANG Jie,WEI Jinyu,QI Jinli,CAO Yupin,LIANG Jingchun,AN Shijia,LIU Xingchen,TENG Jianbei.Study on Quality Standard of the Leaves of Litchi Chinensis Sonn.[J].Chin J Mod Appl Pharm(中国现代应用药学),2023,40(14):1967-1975. |
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| 荔枝叶药材质量标准研究 |
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黄冬芳1, 梁洁1,2,3, 韦金玉1,4, 祁金丽1, 曹玉嫔1, 梁晶春1, 安施佳1, 刘星晨1, 滕建北1
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1.广西中医药大学, 南宁 530200;2.中药固体制剂制造技术国家工程研究中心华南分中心, 南宁 530200;3.广西壮瑶药工程技术研究中心, 南宁 530200;4.广西高校中药提取纯化与质量分析重点实验室, 南宁 530200
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| 摘要: |
| 目的 建立荔枝叶药材的质量标准。方法 建立荔枝叶药材的性状、显微特征、薄层鉴别及含量测定方法；按照中国药典2020年版第四部通则中相关方法，对荔枝叶药材的水分、总灰分、酸不溶性灰分及浸出物等项目进行测定。结果 荔枝叶药材在性状、显微特征和薄层色谱方面均具有专属性特征。药材水分含量为2.66%~6.42%，总灰分含量为2.96%~4.52%，酸不溶性灰分含量为0.17%~0.94%，水溶性浸出物含量为15.38%~19.87%，醇溶性浸出物含量为24.94%~30.33%。没食子酸、原儿茶酸、儿茶素、表儿茶素、芦丁、紫云英苷、槲皮素、山柰酚8种成分分别在0.003 3~0.052 0 mg·mL-1、0.006 9~ 0.109 6 mg·mL-1、0.013 0~0.208 0 mg·mL-1、0.057 5~0.920 0 mg·mL-1、0.013 4~0.213 6 mg·mL-1、0.013 2~0.211 2 mg·mL-1、0.002 9~0.045 6 mg·mL-1、0.003 6~0.056 8 mg·mL-1内呈良好的线性关系(r>0.999)，平均加样回收率97.43%~102.97%，RSD为1.70%~2.90%。结论 建立的荔枝叶药材质量标准具有很好的专属性、准确性、重现性，可用于荔枝叶药材的质量控制研究。 |
| 关键词: 荔枝叶 薄层色谱法 高效液相色谱法 质量标准 |
| DOI：10.13748/j.cnki.issn1007-7693.20221616 |
| 分类号:R284.1 |
| 基金项目:广西高等学校千名中青年骨干教师培育计划(桂教人〔2019〕5号)；广西一流学科建设(桂教科研〔2018〕12号)；首批广西“2011协同创新中心”(桂教科研〔2013〕20号)；广西壮瑶药重点实验室(桂科基字〔2014〕32号)；广西高等学校高水平创新团队及卓越学者计划项目(桂教师范〔2019〕52号)；广西科技基地和人才专项(桂科AD20238058) |
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| Study on Quality Standard of the Leaves of Litchi Chinensis Sonn. |
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HUANG Dongfang1, LIANG Jie1,2,3, WEI Jinyu1,4, QI Jinli1, CAO Yupin1, LIANG Jingchun1, AN Shijia1, LIU Xingchen1, TENG Jianbei1
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1.Guangxi University of Chinese Medicine, Nanning 530200, China;2.South China Branch of National Engineering Research Center for Manufacturing Technology of Traditional Chinese Medicine Solid Preparation, Nanning 530200, China;3.Guangxi Zhuang Yao Medicine Center of Engineering and Technology, Nanning 530200, China;4.Key Laboratory of TCM Extraction and Purification and Quality Analysis, Guangxi Colleges and Universities, Nanning 530200, China
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| Abstract: |
| OBJECTIVE To establish the quality standard of the leaves of Litchi chinensis Sonn.. METHODS The identification of medicinal properties, microscopic characteristics, TLC and content determination method of the leaves of Litchi chinensis Sonn. was conducted. The moisture, total ash, acid insoluble ash and extract of the leaves of Litchi chinensis Sonn. were determined according to Chinese Pharmacopoeia(Volume IV), 2020. RESULTS The leaves of Litchi chinensis Sonn. under forest exhibited specific properties in characteristics, microscopic features and TLC results. The moisture content of the medicinal materials was 2.66%-6.42%, the total ash content was 2.96%-4.52%, the acid insoluble ash content was 0.17%-0.94%, the water soluble extract was 15.38%-19.87%, and the alcohol soluble extract was 24.94%-30.33%. The eight components of gallic acid, protocatechin, catechin, epicatechin, rutin, astragalin, quercetin and kaempferol had a good linear relationship in 0.003 3-0.052 0 mg·mL-1, 0.006 9-0.109 6 mg·mL-1, 0.013 0-0.208 0 mg·mL-1, 0.057 5-0.920 0 mg·mL-1, 0.013 4-0.213 6 mg·mL-1, 0.013 2-0.211 2 mg·mL-1, 0.002 9-0.045 6 mg·mL-1, 0.003 6-0.056 8 mg·mL-1(r>0.999). The average sample recovery rate was 97.43%-102.97% and the RSD was 1.70%-2.90%. CONCLUSION The established quality standard of the leaves of Litchi chinensis Sonn. medicinal material has good specificity, accuracy and reproducibility, which can be used for quality control research of the leaves of Litchi chinensis Sonn.. |
| Key words: leaves of Litchi chinensis Sonn. TLC HPLC quality standard |
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