| 引用本文: | 朱徐东,姚伟,严斐霞,黄东,王松,朱娜,袁铭铭,黄丽萍,吴毅.基于UPLC-Q/TOF-MS技术快速分析樟帮九蒸九晒地黄的化学成分及固体辅料缓解地黄“滋腻碍脾”的功能研究[J].中国现代应用药学,2024,41(10):. |
| zhuxudong,YAO Wei,YAN Feixia,HUANG Dong,WANG Song,ZHU Na,YUAN Mingming,HUANG Liping,WU Yi.Rapid analysis of the chemical composition of Zhangbang Nine Steaming Nine Sun-dring Rehmanniae Radix based on UPLC-Q/TOF-MS technique and Study on the Function of Solid Excipients in Alleviating the “Nourishing and Spleen-impairing” in Rehmanniae Radix[J].Chin J Mod Appl Pharm(中国现代应用药学),2024,41(10):. |
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| 基于UPLC-Q/TOF-MS技术快速分析樟帮九蒸九晒地黄的化学成分及固体辅料缓解地黄“滋腻碍脾”的功能研究 |
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朱徐东1, 姚伟2, 严斐霞2, 黄东3, 王松2, 朱娜2,3, 袁铭铭3, 黄丽萍2, 吴毅3
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1.江西中医药大学，江西省药品检验检测研究院;2.江西中医药大学;3.江西省药品检验检测研究院
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| 摘要: |
| 摘要: 目的 利用UPLC-Q/TOF-MS技术研究樟帮九蒸九晒地黄的化学成分及固体辅料缓解地黄“滋腻碍脾”的功能。方法 本研究采用超高效液相色谱串联四极杆飞行时间质谱仪（UPLC-Q/TOF-MS）对樟帮九蒸九晒地黄进行数据采集，利用UNIFI平台及自建数据库对樟帮九蒸九晒地黄化学成分进行快速定性分析及鉴定；通过建立小鼠胃动力低下模型，测定其胃残留率和小肠推进率，研究固体辅料对地黄“滋腻碍脾”功能的影响。结果 通过UPLC-Q/TOF-MS和UNFI平台鉴定出九蒸九晒地黄中化学成分76个，包括9个环烯醚萜苷类、11个苯乙醇苷类、31个黄酮类、6个糖类化合物、19个其它类化合物；对小鼠胃排空和小肠推进作用实验结果显示，樟帮九蒸九晒地黄具有胃排空和肠推进作用。结论 该方法可用于九蒸九晒地黄的快速鉴别分析，九蒸九晒地黄中固体辅料陈皮、砂仁可以缓解地黄的“滋腻碍脾”功能。本研究以期为樟帮特色饮片九蒸九晒地黄的质量控制和药效物质基础的阐明提供参考。 |
| 关键词: UPLC-Q/TOF-MS 九蒸九晒地黄 滋腻碍脾 化学成分 胃排空 肠推进 |
| DOI：10.13748/j.cnki.issn1007-7693.20223933 |
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| 基金项目:江西省主要学科学术和技术带头人项目(20182BCB22005)；江西省重点研发计划项目（20192BBG70071, 20202BBGL73006）；江西省研究生创新专项资金项目(YC2021-S505)；2023全国中药特色技术传承人才培训项目（T20234832005）。 |
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| Rapid analysis of the chemical composition of Zhangbang Nine Steaming Nine Sun-dring Rehmanniae Radix based on UPLC-Q/TOF-MS technique and Study on the Function of Solid Excipients in Alleviating the “Nourishing and Spleen-impairing” in Rehmanniae Radix |
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zhuxudong,YAO Wei,YAN Feixia,HUANG Dong,WANG Song,ZHU Na,YUAN Mingming,HUANG Liping,WU Yi
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1.Jiangxi University of Chinese medicine;2.Jiangxi Institute for Drug Control
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| Abstract: |
| ABSTRACT: OBJECTIVE The chemical composition and the function of solid excipients in relieving the "nourishing and spleen-impairing" effect of Zhangbang nine steaming nine sun-dring Rehmanniae Radix with the use of UPLC-Q/TOF-MS technique. METHODS In this study, UPLC-Q/TOF-MS was used to collect the data of Zhangbang nine steaming nine sun-dring Rehmanniae Radix, and UNIFI platform and self-built database were used for the rapid qualitative analysis and identification of the chemical components of Zhangbang nine steaming nine sun-dring Rehmanniae Radix. The effect of solid excipients on the function of Rehmanniae Radix " nourishing and spleen-impairing " was investigated by establishing a mouse model of low gastric motility and measuring its gastric residual rate and small intestine propulsion rate. RESULTS The UPLC-Q/TOF-MS and UNFI platforms identified 76 chemical components in nine steaming nine sun-dring Rehmanniae Radix, including 9 cyclic enol ether glycosides, 11 phenylethyl glycosides, 31 flavonoids, 6 saccharides and 19 other compounds; the results of gastric emptying and small intestinal propulsion experiments on mice showed that Zhangbang nine steaming nine sun-dring Rehmanniae Radix had gastric emptying and intestinal propulsion effects. CONCLUSIONS This method can be used for the rapid identification and analysis of nine steaming nine sun-dring Rehmanniae Radix, which has solid excipients pericarpium citri reticulatae and fructus amomi to alleviate the " nourishing and spleen-impairing" function of Rehmanniae Radix. This study is intended to provide a reference for quality control and elucidation of the material basis of the medicinal effects of specialty beverage of Zhangbang nine steaming nine sun-dring Rehmanniae Radix. |
| Key words: UPLC-Q/TOF-MS nine steaming nine sun-dring Rehmanniae Radix nourishing and spleen-impairing chemical composition gastric emptying intestinal propulsion |
