基于UPLC-Q-TOF-MS/MS的紫地榆化学成分分析

Identification of Components in Geranium Strictipes Knuth based on UPLC-Q-TOF-MS/MS

关键词:  紫地榆  UPLC-Q-TOF-MS/MS  化学成分

Key words:  Geranium Strictipes Knuth  UPLC-Q-TOF-MS/MS  chemical composition

摘要:

目的 基于超高效液相色谱-四极杆飞行时间质谱(UPLC-Q-TOF-MS/MS)分析鉴定紫地榆中的化学成分。方法 色谱条件:采用ACQUITY UPLC-BEH C18色谱柱(2.1 mm×100 mm,1.7 μm),以乙腈(A)-0.1%甲酸溶液(B)为流动相,进行梯度洗脱；质谱条件:采用ESI电喷雾离子源,进行正、负离子全扫描。结果 通过对质谱数据分析并参考相关文献,结合对照品比对结果,共鉴定出67个化学成分,包含鞣质类20个、有机酸类21个、黄酮及其苷类15个、萜类11个。结论 本研究建立的方法快速、准确,可为紫地榆的质量控制研究及物质基础研究提供参考依据。

Abstract:

ABSTRACT: OBJECTIVE To identify the chemical constituents in Geranium Strictipes Knuth based on ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS/MS). METHODS Chromatographic conditions: using ACQUITY UPLC-BEH C18 column (2.1 mm×100 mm, 1.7 μm), using acetonitrile (A)-0.1% formic acid solution (B) as mobile phase for gradient elution; mass spectrometry conditions: using ESI electron Spray ion source for full scan of positive and negative ions. RESULTS By analyzing the mass spectrometry data and referring to relevant literature, combined with the comparison results of reference substances, a total of 67 chemical components were identified, including 20 tannins, 21 organic acids, 15 flavonoids and their glycosides, and 11 terpenes. CONCLUSION The rapid and accurate establishment of this study can provide a reference for the control research and material basis research of Geranium Strictipes Knuth. KEYWORDS: Geranium Strictipes Knuth; UPLC-Q-TOF-MS/MS; chemical composition