| 引用本文: | 廖荣俊,颜晓捷,王捷希,徐墨池,张春椿,秦路平,朱波.多花黄精可培养内生真菌菌群结构、多样性与分布规律[J].中国现代应用药学,2025,42(24):103-109. |
| LIAO Rongjun,YAN Xiaojie,WANG Jiexi,XU Mochi,Zhang Chunchun,QIN Luping,ZHU Bo.Community, Diversity, and Distribution Pattern of Culturable Endophytic Fungi Isolated from Polygonatum cyrtonema[J].Chin J Mod Appl Pharm(中国现代应用药学),2025,42(24):103-109. |
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| 多花黄精可培养内生真菌菌群结构、多样性与分布规律 |
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廖荣俊1, 颜晓捷2, 王捷希3, 徐墨池3, 张春椿3, 秦路平3, 朱波3
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1.衢州市森林资源保护中心;2.衢州市乌溪江饮用水源保护管理中心;3.浙江中医药大学
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| 摘要: |
| 目的 分离多花黄精内生真菌,分析其菌群结构、多样性与分布规律。方法 将采集的多花黄精健康植株分为根、茎、叶、根茎4个组织部位,用传统分离法分离不同组织部位内生真菌,用ITS序列进行分子鉴定,分析菌群结构与多样性。结果 共分离得到多花黄精可培养内生真菌184株,根据菌落特征及分子鉴定划分为45个分类单元,归属到1个门2个纲6个目9个科10个属；多花黄精内生真菌分布存在组织差异性与特异性,其中木霉属Trichoderma为根、茎、叶中最优势属,螺旋聚孢霉属Clonostachys为根茎的专有属；Shannon-Wiener多样性(H’)和Simpson多样性(1-D)分析显示,多样性指数呈现根>茎>叶>根茎的趋势；相似性分析显示,根和茎内生真菌菌群相似度最高,相似性指数Cs为0.6885。结论 多花黄精具有丰富的内生真菌资源,为内生真菌生物防治提供了备选菌株,也为多花黄精内生真菌资源开发与利用提供了基础资料。 |
| 关键词: 多花黄精 内生真菌 组织部位 菌群结构 多样性 分布规律 |
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| Community, Diversity, and Distribution Pattern of Culturable Endophytic Fungi Isolated from Polygonatum cyrtonema |
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LIAO Rongjun1, YAN Xiaojie2, WANG Jiexi3, XU Mochi3, Zhang Chunchun3, QIN Luping3, ZHU Bo3
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1.Quzhou Forest Resources Conservation Center;2.Quzhou Wuxi River Potable Water Sources Protection and Management Center;3.Zhejiang Chinese Medical University
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| Abstract: |
| ABSTRACT: OBJECTIVE To isolate culturable endophytic fungi from Polygonatum cyrtonema and analyze their community, diversity, and distribution pattern. METHODS Several healthy P. cyrtonema individuals were collected and divided into four parts, including root, stem, leaf, and rhizome. Culturable endophytic fungi were isolated from these parts using traditional isolation techniques. ITS sequence was used to identify the fungal isolates, and their fungal community and diversity were analyzed. RESULTS A total of 184 endophytic fungal strains were isolated and divided into 45 taxons, belonging to 1 phylum, 2 classes, 6 orders, 9 families, and 10 genera. There were tissue differences and specificities in the distribution of endophytic fungi. Among them, Trichoderma topped the dominant genera of those isolated from roots, stems, and leaves, while Clonostachys was only isolated from rhizome. Shannon-Wiener diversity (H’) and Simpson diversity (1-D) analysis showed that root had the highest diversity index, followed by stem, leaf, and rhizome. There was the highest similarity between root and stem with the Cs index was 0.6885. CONCLUSIONS There are abundant endophytic fungi in various P. cyrtonema tissues, The paper provided candidate strain for the endophytic fungal biological control, and the basic data for the development and utilization of endophytic fungi resources in P. cyrtonema. |
| Key words: Polygonatum cyrtonema endophytic fungi tissues fungal community diversity distribution pattern |
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