| 引用本文: | 周日花,郑小玲,王顺强,何跃芳.实时荧光定量PCR快速检测技术在制药用水洋葱伯克霍尔德菌群检测中的替代应用研究[J].中国现代应用药学,2025,42(23):144-151. |
| zhourihua,zhengxiaoling,wangshunqiang,heyuefang.Application of real-time fluorescent quantitative PCR for rapid detection as an alternative method in the detection of Burkholderia cepacia complex in pharmaceutical water[J].Chin J Mod Appl Pharm(中国现代应用药学),2025,42(23):144-151. |
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| 摘要: |
| 目的 验证实时荧光定量PCR快速检测技术替代制药用水洋葱伯克霍尔德菌群（Burkholderia cepacia complex,Bcc）检查增菌培养后检测的可行性。 方法 先采用35株伯克霍尔德菌属微生物对该属的增菌培养基未稀释的胰酪大豆胨液体培养基（Tryptic Soy Broth, TSB）培养基和稀释10倍的TSB培养基开展增菌效果的比较研究。后采用基于实时荧光定量PCR技术的全自动核酸扩增检测系统，对制药用水洋葱伯克霍尔德菌群检验增菌培养后的替代方法开展专属性、检测限、耐用性和重现性四个参数的系统验证研究。 结果 研究结果表明稀释10倍的TSB培养基对Bcc类的伯克霍尔德菌属微生物的增菌效果优于未稀释的TSB培养基。本研究中的替代方法的检测限结果与药典培养法一致，专属性、耐用性和重现性结果均较好。 结论 稀释10倍的TSB培养基更适合Bcc类的伯克霍尔德菌属微生物的增菌培养。通过替代验证研究表明实时荧光定量PCR快速检测技术适用于洋葱伯克霍尔德菌群检查增菌培养后的快速检测。 |
| 关键词: 制药用水 洋葱伯克霍尔德菌 实时荧光PCR检测技术 快速检测 替代验证 |
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| 基金项目:浙江省市场监督管理局2025年度科技计划项目(项目编号ZC2025018) |
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| Application of real-time fluorescent quantitative PCR for rapid detection as an alternative method in the detection of Burkholderia cepacia complex in pharmaceutical water |
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zhourihua1, zhengxiaoling2, wangshunqiang3, heyuefang3
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1.Zhejiang Integrated Traditional Chinese and Western Medicine Hospital;2.Zhejiang Institute for Food and Drug Control;3.Zhejiang Toyouvax Biopharming
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| Abstract: |
| ABSTRACT：OBJECTIVE To verify the feasibility of using real-time fluorescent quantitative PCR (qPCR) for rapid detection as an alternative to the post-enrichment detection method for Burkholderia cepacia complex (Bcc) in pharmaceutical water. METHODS Firstly, a comparative study on the enrichment efficacy of undiluted Tryptic Soy Broth (TSB) medium and 10-fold diluted TSB medium for Burkholderia species was conducted using 35 strains of Burkholderia microorganisms. Subsequently, a fully automated nucleic acid amplification detection system based on qPCR technology was employed to systematically validate the specificity, limit of detection (LOD), robustness, and reproducibility of the alternative method for post-enrichment detection of Bcc in pharmaceutical water. RESULTS The results indicated that the 10-fold diluted TSB medium was superior to the undiluted TSB medium for the enrichment of Bcc strains within the Burkholderia genus. The LOD of the alternative method in this study was consistent with that of the pharmacopoeia culture method, and the results for specificity, robustness, and reproducibility were all satisfactory. CONCLUSION The 10-fold diluted TSB medium is more suitable for the enrichment culture of Bcc strains within the Burkholderia genus. The validation study demonstrated that qPCR is suitable for rapid detection of Bcc in pharmaceutical water after enrichment culture. |
| Key words: pharmaceutical water Burkholderia cepacia qPCR rapid method alternative validation |
