近红外漫反射光纤光谱结合偏最小二乘法快速测定黑果小檗皮中盐酸小檗碱含量

Rapid Content Petermination of Berberine Hydrochloride in Berberis Heteropoda Skin by Near Infrared Diffuse Reflection Optical Spectra Combined with Partial Least Squares

关键词:  黑果小檗  近红外光谱分析技术  偏最小二乘法

Key words:  Berberis heteropoda Schrenk  near infrared reflectance spectroscopy(NIRS)  partial least-squares regression (PLSR)

摘要:

目的 应用近红外漫反射光纤光谱结合偏最小二乘法(NIR-PLSR)对黑果小檗皮中盐酸小檗碱进行快速定量分析。方法 建立NIR-PLSR数学模型对黑果小檗皮中盐酸小檗碱进行快速定量分析。结果 黑果小檗皮中盐酸小檗碱的内部交叉验证均方差(RMSECV)为0.810 2，内部交叉验证决定系数为0.987 1，预测平均回收率为101.4%，统计学检验表明NIR预测值与标准方法测定值之间的差异无统计学意义。结论 利用NIR-PLSR对黑果小檗进行定量分析可行。

Abstract:

OBJECTIVE To analysis berberine hydrochloride in Berberis heteropoda skin combined near infrared diffuse reflection optical spectra with partial least squares(NIR-PLSR). METHODS Establish a method of NIR diffuse reflectance optic-fiber spectroscopy combine with PLSR to analysis berberine hydrochloride in Berberis heteropoda skin. RESULTS The root-mean-square error of cross-validation(RMSECV) of the berberine hydrochloride in Berberis heteropoda skin was 0.810 2, the correlation coefficients was 0.987 1, the average recycle rate was 101.4%. Statistical test was used to analysis the dates and it showed that the difference between NIR prediction values and the values determinated by standard methods had no statistical significance. CONCLUSION It indicates that NIR diffuse reflectance optic-fiber spectroscopy combined with PLSR method could be used in rapid determination of the content of berberine hydrochloride in Berberis heteropoda Schrenk.