附子白芍配伍对佐剂性关节炎大鼠的抗炎活性研究

Study of Anti-inflammatory Activity of Compatibility of Aconiti Lateralis Radix Praeparata and Paeoniae Radix Alba on the Adjuvant Arthritis Rats

关键词:  附子  白芍  配伍  抗炎活性

Key words:  Aconiti Lateralis Radix Praeparata  Paeoniae Radix Alba  compatibility  anti-inflammatory activity

摘要:

目的 观察附子白芍配伍前后对佐剂性关节炎模型大鼠抗炎活性的影响。方法 采用完全弗氏佐剂诱导建立佐剂性关节炎大鼠炎性痛模型，随机分为模型组、附子组、白芍组、附子白芍配伍组，以雷公藤多苷为阳性药，另设正常组。分别测定各组大鼠足跖肿胀体积，ELISA法测定血清中PGE2、IL-1β和TNF-α浓度。结果 与模型组比较，雷公藤多苷组、附子组、白芍组，配伍组均能够降低大鼠左右后足的肿胀度以及血清中的PGE2、IL-1β和TNF-α浓度，其中附子白芍配伍组、雷公藤多苷组、白芍组作用最强(P<0.01)，附子组和白芍组均弱于配伍组，附子组的抑制作用最弱，与配伍组有显著统计学差异(P<0.01)。结论 附子、白芍均有不同程度的抗炎活性，两者配伍能显著增强各单味药尤其是附子的抗炎活性，表明两者配伍具有明显的协同增效作用。

Abstract:

OBJECTIVE To investigate the anti-inflammatory effect of Aconiti Lateralis Radix Praeparata combined with Paeoniae Radix Alba on the arthritic rat. METHODS The rat model was established by injecting the complete Frund’s adjuvant into the subcutis of their left rear paws. The rats were randomly divided into control group, model group, tripterygium glycosides group, Aconiti Lateralis Radix Praeparata group, Paeoniae Radix Alba group and combinational group(treated with Aconiti Lateralis Radix Praeparata and Paeoniae Radix Alba). Meanwhile, the volume of paw swelling was measured, and the serum levels of PGE2, IL-1β and TNF-α were detected by ELISA. RESULTS Compared with model group, the tripterygium glycosides group, Aconiti Lateralis Radix Praeparata group, Paeoniae Radix Alba group and combinational group could decrease the swelling degree of the rat paws and decrease the serum concentration of PGE2, IL-1β,TNF-α. And the combinational group, tripterygium glycosides group, Paeoniae Radix Alba group had the strongest effect (P<0.01), Aconiti Lateralis Radix Praeparata group and Paeoniae Radix Alba group were weaker than the combination group, the weakest inhibitory action of aconite group, with significant difference with compound group(P<0.01). CONCLUSION Both of Aconiti Lateralis Radix Praeparata and Paeoniae Radix Alba treatment group have obviously anti-inflammation activity. the combination treatment can significantly enhance the anti-inflammation activity of any single one. It may indicate that synergy effects are generated during the combinational treatment of the two herb.