| 引用本文: | 龚俊强,蒋彩霞,陈仙,章小永,陶灵芝,白海波,张霞.黄杞叶黄酮类成分的研究[J].中国现代应用药学,2015,32(7):813-817. |
| GONG Junqiang,JIANG Caixia,CHEN Xian,ZHANG Xiaoyong,TAO Lingzhi,BAI Haibo,ZHANG Xia.Studies on Flavonoids from the Leaves of Engelhardia Roxburghiana[J].Chin J Mod Appl Pharm(中国现代应用药学),2015,32(7):813-817. |
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| 黄杞叶黄酮类成分的研究 |
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龚俊强1,2, 蒋彩霞3, 陈仙2, 章小永4, 陶灵芝4, 白海波4, 张霞4
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1.浙江大学药学院，杭州 310058;2.浙江华义医药有限公司，浙江 义乌 322002;3.中美华东制药有限公司，杭州 310011;4.华东医药生物工程研究所，杭州 310011
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| 摘要: |
| 目的 研究黄杞叶中的黄酮类成分。方法 综合采用硅胶、Sephadex LH-20柱色谱及制备型高效液相色谱法，对黄杞叶中的黄酮类成分进行分离纯化，根据理化性质并结合UV、HPLC/Q-TOF-ESI-MS、1H-NMR、13C-NMR、DEPT、HMBC数据及文献进行结构鉴定。结果 分得了6个化合物，分别鉴定为落新妇苷(化合物Ⅰ)、黄杞苷(化合物Ⅱ)、槲皮苷(化合物Ⅲ)、(2R, 3R)5, 7, 3', 4'-四羟基二氢黄酮-3-氧-α-L-鼠李糖(3→1)-β-D-葡萄糖苷(化合物Ⅳ)、(2R, 3R)5, 7, 4'-三羟基二氢黄酮-3-氧-α-L-鼠李糖(3→1)-β-D-葡萄糖苷(化合物Ⅴ)、(2R, 3R)-5, 7, 4', 5'-四羟基二氢黄酮-3-氧-(3"-氧-没食子酰基)-α-L-鼠李糖苷(化合物Ⅵ)。结论 化合物Ⅴ为首次从该植物中得到，也是新化合物。 |
| 关键词: 黄杞 黄酮 柱色谱 制备型高效液相色谱 |
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| Studies on Flavonoids from the Leaves of Engelhardia Roxburghiana |
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GONG Junqiang1,2, JIANG Caixia3, CHEN Xian2, ZHANG Xiaoyong4, TAO Lingzhi4, BAI Haibo4, ZHANG Xia4
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1.College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China;2.Zhejiang Huayi Pharmaceutical Co., Ltd., Yiwu 322002, China;3.Zhongmei Huadong Pharmaceutical Co., Ltd., Hangzhou 310011, China;4.Biological Engineering Research Institute, Hangzhou Huadong Medicine Group, Hangzhou 310011, China
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| Abstract: |
| OBJECTIVE To study the flavonoids from the leaves of Engelhardia roxburghiana for better using the traditional Chinese medicine. METHODS The flavonoids was isolated and purified by silica gel, Sephadex LH-20 column chromatography, preparative HPLC and the structures were elucidated on the basis of spectral data including UV, HPLC/Q-TOF-ESI-MS, 1H-NMR, 13C-NMR, DEPT, HMBC and physiochemical properties. RESULTS Six flavonoids were obtained from the 95% ethanol extract and their structures were identified as astilbin (Ⅰ), engelitin (Ⅱ), quercitrin (Ⅲ), (2R, 3R)5,7,3’,4’-tetrahydroxyflavanonol-3-O-α-L-rhamnopyranosyl(3→1)-β-D-glucopyranoside (Ⅳ), (2R, 3R) 5, 7, 4'-trihydroxyfla- vanonol-3-O-α-L-rhamnopyranosyl (3→1)-β-D-glucopyranoside (Ⅴ), (2R, 3R)-5, 7, 4', 5'- tetrahydroxyflavanonol-3-O-(3"-O- galloyl)-α-L-rhamnopyranoside (Ⅵ), respectively. CONCLUSION Compound Ⅴ is first isolated from the title plant and reported for the first time. |
| Key words: Engelhardia roxburghiana flavonoid column chromatography preparative HPLC |
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