HPLC同时测定黄石感冒片中阿魏酸、芦丁、大黄酸、大黄素和大黄酚的含量

Simultaneous Determination of Ferulic Acid, Rutin, Rhein, Emodin and Chrysophanol in Huangshiganmao Tablets by HPLC

关键词:  黄石感冒片  阿魏酸  芦丁  大黄酸  大黄素  大黄酚  HPLC

Key words:  Huangshiganmao tablets  ferulic acid  rutin  rhein  emodin  chrysophanol  HPLC

摘要:

目的 建立HPLC同时测定黄石感冒片中阿魏酸、芦丁、大黄酸、大黄素和大黄酚的含量。方法 采用HPLC，色谱柱为Welch Topsil-C₁₈柱(4.6 mm×250 mm，4 μm)；以甲醇-0.1%磷酸溶液(78∶22)为流动相；流速：1.0 mL·min^-1；柱温：30 ℃；检测波长：280 nm。结果 阿魏酸、芦丁、大黄酸、大黄素和大黄酚峰线性范围分别为0.020 82~0.416 3 μg·mL^-1(r=0.999 7)，0.011 32~0.278 3 μg·mL^-1(r=0.999 3)，0.017 22~0.344 3 μg·mL^-1(r=0.999 5)，0.015 79~0.315 8 μg·mL^-1(r=0.999 6)和0.051 34~1.027 μg·mL^-1(r=0.999 9)，平均加样回收率分别为97.4%(RSD=1.1%)，95.0%(RSD=0.88%)，97.5%(RSD=1.3%)，97.4%(RSD=1.4%)和96.3%(RSD=0.87%)。结论 新建方法简便、准确，可用于黄石感冒片的定量分析方法。

Abstract:

OBJECTIVE To establish an HPLC method for the simultaneous determination of ferulic acid, rutin, rhein, emodin and chrysophanol in Huangshiganmao tablets. METHODS A Welch Topsil-C₁₈(4.6 mm×250 mm, 4 μm)column was used with the mobile phase of methanol-0.1% phosphate solution(78∶22) at a flow rate of 1.0 mL·min^-1, the column temperature of 30 ℃ and the detection wavelength was 280 nm. RESULTS The calibration curves of ferulic acid, rutin, rhein, emodin and chrysophanol were linear over the ranges of 0.020 82-0.416 3 μg·mL^-1(r=0.999 7), 0.011 32-0.278 3 μg·mL^-1(r=0.999 3), 0.017 22-0.344 3 μg·mL^-1(r=0.999 5), 0.015 79-0.315 8 μg·mL^-1(r=0.999 6) and 0.051 34-1.027 μg·mL^-1(r=0.999 9) respectively, and the average recoveries were 97.4% (RSD=1.1%), 95.0% (RSD=0.88%), 97.5% (RSD=1.3%), 97.4% (RSD=1.4%) and 96.3%(RSD=0.87%) respectively. CONCLUSION This method was simple, accurate and can be used for quantitative analysis method of Huangshiganmao tablets.