HPLC同时测定丹绿补肾胶囊中次野鸢尾黄素和胡椒碱的含量

Simultaneous Determination of Irisflorentin and Piperine in Danlyu Bushen Capsules by HPLC

关键词:  丹绿补肾胶囊  次野鸢尾黄素  胡椒碱  高效液相色谱法  含量测定

Key words:  Danlyu Bushen capsules  irisflorentin  piperine  HPLC  content determination

摘要:

目的 建立同时测定丹绿补肾胶囊中次野鸢尾黄素、胡椒碱含量的HPLC方法。方法 选用Agilent ZORBAX Eclipse Plus C₁₈色谱柱（4.6 mm×250 mm，5 μm），乙腈-0.2%磷酸为流动相（41∶59），流速为1.0 mL·min^-1，柱温为40℃，检测波长为266 nm。结果 次野鸢尾黄素和胡椒碱分别在0.010 30~0.309 0 μg，0.081 73~2.451 9 μg内呈良好的线性关系，相关系数≥0.999 8；加样回收率（n=9）分别为99.7%，100.6%；RSD分别为1.2%，0.9%；样品在24 h内稳定，次野鸢尾黄素和胡椒碱RSD分别为1.6%，0.6%；在测定样品中，次野鸢尾黄素和胡椒碱最低含量分别为每粒0.35，6.4 mg。结论 该方法简便、准确、重复性好，可作为丹绿补肾胶囊的质量控制方法。

Abstract:

OBJECTIVE To establish an HPLC method for the simultaneous determination of irisflorentin and piperine in Danlyu Bushen capsules. METHODS The HPLC was carried on Agilent ZORBAX Eclipse Plus C₁₈(4.6 mm×250 mm, 5 μm), the mobile phase acetonitrile-0.2% phosphoric acid (41:59), the flow rate was 1.0 mL·min^-1, the column temperature was 40℃, detection wavelength was 266 nm. RESULTS Under the chromatographic conditions, irisflorentin in 0.010 30-0.309 0 μg (r=0.999 8); piperine in 0.081 73-2.451 9 mg (r=0.999 8), had good linear relationship. Irisflorenti and piperine average sample recovery rate (n=9) were 99.7% and 100.6%; RSD were 1.2%, 0.9%, respectively. In the sample, the minimum content of irisflorentin and piperine was 0.35, 6.4 mg per granule, respectively. CONCLUSION The method is simple, accurate and has a good repeatability. It can be used as the quality control method of Danlyu Bushen capsules.