百里醌对大鼠肾脏缺血再灌注损伤的保护作用

Protective Effects of Thymoquinone on Renal Ischemia-reperfusion Injury in Rats

关键词:  百里醌  缺血再灌注损伤  肾脏

Key words:  thymoquinone  ischemia reperfusion injury  kidney

摘要:

目的 观察百里醌对大鼠肾缺血再灌注损伤（ischemia-reperfusion injury，IRI）的保护作用。方法 60只健康SD大鼠，♂，随机分成正常组、模型组和百里醌组。模型组和百里醌组大鼠建立肾脏缺血再灌注模型，百里醌组在再灌注同时腹腔注射百里醌（40 mg·kg^-1）。HE染色观察肾脏组织病理改变；测定血清指标肌酐（Scr）、尿素氮（BUN）以及肾组织指标丙二醛（MDA）、超氧化物岐化酶（SOD）；采用原位缺口末端标记检测肾小管上皮细胞凋亡指数（AI）；RT-PCR检测肾脏组织中的caspase-3，Bax和TNF-α和IL-1β的表达情况。结果 模型组可见肾小管扩张，肾小管上皮细胞坏死等，经百里醌治疗后明显改善。与模型组相比，百里醌组凋亡指数明显降低（P<0.05）。与正常组比较，模型组SOD活性显著降低（P<0.05），MDA含量、Scr和BUN显著升高（P<0.05）；与模型组比较，百里醌组SOD活性显著升高（P<0.05），MDA含量、Scr和BUN显著降低（P<0.05）；与正常组比较，模型组中caspase-3、Bax、TNF-α和IL-1β mRNA表达水平明显增高（P<0.05）。和模型组比较，百里醌组中caspase-3、Bax、TNF-α和IL-1β表达明显减低（P<0.05）。结论 百里醌可减轻氧化应激水平，发挥抗炎及抗凋亡作用，从而在肾脏IRI过程中发挥保护作用。

Abstract:

OBJECTIVE To investigate the protective effect of thymoquinone on renal ischemia reperfusion injury in rats.METHODS Sixty male SD rats were randomly divided into three groups:normal group, model group and thymoquinone group. Renal ischemia reperfusion model was established in model group and thymoquinone group, thymoquinone (40 mg·kg^-1) was injected intraperitoneally followed reperfusion in thymoquinone group. The pathological changes of the kidney were observed by HE staining. MDA, SOD, Scr and BUN content were determined; cell apoptosis index was detected by dUTP nick end labeling. The expression of caspase-3, Bax, TNF-α and IL-1β in renal tissue was detected by RT-PCR.RESULTS Model group showed renal tubular expansion, renal tubular epithelial cell necrosis, thymoquinone treatment significantly improved the above changes. Compared with normal group, apoptosis index in model group significantly decreased(P<0.05). Compared with normal group, SOD activities in model group significantly decreased(P<0.05), MDA content, Scr and BUN significantly increased(P<0.05); compared with model group, SOD activities in thymoquinone group significantly increased(P<0.05), MDA content, Scr and BUN significantly decreased(P<0.05). Compared with normal group, the mRNA expression of caspase-3, Bax, TNF-α and IL-1β were significantly increased(P<0.05). Compared with model group, the expression of caspase-3, Bax, TNF-α and IL-1β decreased in thymoquinone group(P<0.05).CONCLUSION Thymoquinone reduces the level of oxidative stress and inhibit the apoptosis and inflammation. Thus, it plays a protective role in the process of renal ischemia-reperfusion injury.