| 引用本文: | 银艳桃,王建超,廖娟,玉叶,朱朝阳,邓鑫.肝宁方含药血清对人肝星状细胞增殖及凋亡的影响[J].中国现代应用药学,2020,37(3):269-274. |
| YIN Yantao,WANG Jianchao,LIAO Juan,YU Ye,ZHU Chaoyang,DENG Xin.Effect of Ganning Formula on Proliferation and Apoptosis of Human Hepatic Stellate Cells[J].Chin J Mod Appl Pharm(中国现代应用药学),2020,37(3):269-274. |
|
| 摘要: |
| 目的 研究肝宁方含药血清对人肝星状细胞（HSC-LX2）增殖及凋亡的影响。方法 40只SD大鼠随机分为4组，每组10只，空白对照组与TGF-β1组SD大鼠给予生理盐水灌胃，TGF-β1+肝宁方组及肝宁方组SD大鼠给予肝宁方灌胃给药（7 mL·kg-1·d-1），连续灌胃10 d后取血离心，制备干燥血清粉末备用。将HSC-LX2分为空白对照组、TGF-β1组、TGF-β1+肝宁方组及肝宁方组。根据分组加入相应的20%大鼠血清对HSC-LX2进行培养造模。CCK8检测各组HSC-LX2细胞增殖率；流式细胞术检测各组HSC-LX2细胞的周期和细胞凋亡；qRT-PCR以及Western blotting分别检测各组α-SMA、Samd2、Samd3、Samd4、Samd7 mRNA及蛋白的表达。结果 CCK8结果显示，TGF-β1+肝宁方组及肝宁方组HSC-LX2增殖受到抑制；流式细胞术结果显示，TGF-β1+肝宁方组及肝宁方组HSC-LX2周期停滞在G0/G1期并能促进细胞凋亡；qRT-PCR及Western blotting结果显示，TGF-β1+肝宁方组及肝宁方组α-SMA、Samd2、Samd3、Samd4 mRNA及蛋白的表达受到抑制，Samd7 mRNA及蛋白的表达增强。结论 肝宁方具有抑制HSC-LX2增殖、促进HSC-LX2凋亡的作用，其作用与调控TGF-β1/Samd信号通路相关。 |
| 关键词: 肝宁方 肝星状细胞 增殖 凋亡 TGF-β1/Samd |
| DOI：10.13748/j.cnki.issn1007-7693.2020.03.003 |
| 分类号:R285.5 |
| 基金项目:国家科技重大专项（2018ZX10303501）；国家自然科学基金项目（81860790，81360532）；广西医疗卫生适宜技术研究与开发项目（S201503）；广西研究生教育创新课题（xjyb151） |
|
| Effect of Ganning Formula on Proliferation and Apoptosis of Human Hepatic Stellate Cells |
|
YIN Yantao1, WANG Jianchao1, LIAO Juan1, YU Ye1, ZHU Chaoyang2, DENG Xin1,2
|
|
1.Guangxi University of Chinese Medicine, Nanning 530000, China;2.Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine, Nanning 530011, China
|
| Abstract: |
| OBJECTIVE To study the effect of Ganning formula on proliferation and apoptosis of human hepatic stellate cells(HSC-LX2). METHODS Forty SD rats were randomly divided into 4 groups, 10 in each group. The blank control group and the TGF-β1 group were given normal saline gavage, and the TGF-β1+Ganning formula group and Ganning formula group were given gavage(7 mL·kg-1·d-1). HSC-LX2 was divided into blank control group, TGF-β1 group, TGF-β1+Ganning formula group and Ganning formula group. The corresponding rat serum was added to the corresponding groups according to the data for the culture and modeling of HSC-LX2. The proliferation of HSC-LX2 cells in each group was detected by CCK8. Flow cytometry was used to detect the cell cycle and apoptosis of HSC-LX2 cells in each group. The mRNA and protein expressions of α-SMA, Samd2, Samd3, Samd4 and Samd7 were detected by qRT-PCR and Western blotting. RESULTS The results of CCK8 showed that the proliferation of HSC-LX2 was inhibited in the serum group containing Ganning formula. The results of flow cytometry showed that the cycle of HSC-LX2 in the G0/G1 phase of the serum group containing Ganning formula was stagnated and cell apoptosis was promoted. qRT-PCR and Western blotting results showed that the mRNA and protein expressions of α-SMA, Samd2, Samd3 and Samd4 were inhibited and the mRNA and protein expressions of Samd7 were enhanced. CONCLUSION Ganning formula can inhibit the proliferation of HSC-LX2 and promote the apoptosis of HSC-LX2, which may be related to the regulation of TGF-β1/Samd signaling pathway. |
| Key words: Ganning formula hepatic stellate cells proliferation apoptosis TGF-β1/Samd |
