| 引用本文: | 李喜凤,郝哲,邱天宝,余云辉,杜云锋.HPCE同时测定蒲公英3种有机酸的含量[J].中国现代应用药学,2011,28(4):350-353. |
| LI Xifeng,HAO Zhe,QIU Tianbao,YU Yunhui,DU Yunfeng.Determination of Three Phenolic Acid in Dandelion by HPCE[J].Chin J Mod Appl Pharm(中国现代应用药学),2011,28(4):350-353. |
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| 摘要: |
| 目的 建立蒲公英中主要有效成分咖啡酸、绿原酸和阿魏酸的高效毛细管电泳含量测定方法。方法 电泳条件：未涂层的弹性石英毛细管柱(75 μm×57 cm)，电解质为20 mmol·L-1硼砂溶液(pH 9.55)，分离电压18 kV，柱温25 ℃，压力进样5 s，检测波长325 nm。结果 咖啡酸、绿原酸、阿魏酸分别在0.010 2~0.081 8 mg?mL-1，0.008 1~0.065 0 mg·mL-1，0.000 6~0.004 5 mg·mL-1内呈良好线性关系，平均回收率分别98.8%，98.9%，97.8%，RSD分别为1.12%，1.21%，1.46%。结论 本法专属性强，简便快速，准确度高，重复性好，适用于检测蒲公英中咖啡酸、绿原酸和阿魏酸的含量。 |
| 关键词: 蒲公英 咖啡酸 绿原酸 阿魏酸 高效毛细管电泳法 |
| DOI： |
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| 基金项目:河南省科技厅重点科技攻关资助项目(0623060100) |
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| Determination of Three Phenolic Acid in Dandelion by HPCE |
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LI Xifeng1, HAO Zhe2, QIU Tianbao1, YU Yunhui1, DU Yunfeng1
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1.Henan University of TCM, Zhengzhou 450008, China;2.The PLA 153 Central Hospital, Zhengzhou 450042, China
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| Abstract: |
| OBJECTIVE To establish a method for determining the contents of caffeic acid(CA), chlorogenic acid(CGA) and ferulic acid(FA) in dandelion by HPCE. METHODS Dandelion was determined at 325 nm using HPCE, under the optimized conditions achieved with an elastic fused-sillia capillary tube(75 μm×57 cm) and 20 mmol·L-1 borate running buffer solution (pH 9.55) at a constant voltage of 18 kV and injecting pressure time of 5 s at 25 ℃. RESULTS The calibration curve displayed good linear relationship within CA, CGA, FA concentration range of 0.010 2-0.081 8 mg·mL-1, 0.008 1-0.065 0 mg·mL-1, 0.000 6-0.004 5 mg·mL-1. The average recovery was 98.8%, 98.9%, 97.8%, RSD was 1.12%, 1.21%, 1.46%, respectively. CONCLUSION This method is simple, rapid, accurate and good repeatability in separation and determination of CA, CGA, FA in dandelion. |
| Key words: dandelion caffeic acid chlorogenic acid ferulic acid HPCE |
