不同产地、不同年限人参中淀粉酶、酯酶、酸性磷酸酯酶的活力比较

Comparison of Activities of Amylase, Esterase, Acid Phosphatase in Ginseng Radix et Rhizoma from Different Origins and Different Growth Periods

关键词:  人参  淀粉酶  酯酶  酸性磷酸酯酶

Key words:  Ginseng Radix et Rhizoma  amylase  esterase  acid phosphatase

摘要:

目的 对不同产地、不同生长年限人参进行淀粉酶(AMY)、酯酶(Est)和酸性磷酸酯酶(ACP)活力比较。方法 用中性缓冲溶液提取总蛋白，应用3,5-二硝基水杨酸比色法测定AMY活力；乙酸-α-萘酯比色法测定Est活力；对硝基酚比色法测定ACP活力。结果 不同产地人参AMY、Est、ACP活力均存在很大差异。取不同产地、相同生长年限酶活力平均值进行不同年限的酶活力比较，可知4年与5年生人参3种水解酶活力均无明显差异。结论 AMY、Est、ACP活力可以作为评价人参质量的内在指标之一。

Abstract:

OBJECTIVE To compare activities of Ginseng Radix et Rhizoma amylase(AMY), esterase (Est) and acid phosphatase (ACP) from different origins and different growth periods. METHODS Total protein was extracted using a neutral buffer solution. Determination of AMY activity applied 3,5-dinitrosalicylic acid colorimetry. Determination of Est activity was using acetic acid-α-naphthyl ester colorimetry. Determination of ACP activity was using p-nitrophenol colorimetry. RESULTS There was a significant difference in activities of AMY, Est and ACP in Ginseng Radix et Rhizoma from different origins. Take the average value for enzyme activity in Ginseng Radix et Rhizoma of different origin and the same growth period, purpose was to compare the enzyme activity at different ages. We can know that activities of three kinds of enzyme hydrolysis were not significantly different in Ginseng Radix et Rhizoma from 4 years old and 5 years old. CONCLUSION Activities of AMY, Est, ACP can be used as one of the intrinsic indicators to assess the quality of Ginseng Radix et Rhizoma.