不同产地黄连中6个生物碱含量测定

Determination of the Content of Six Alkaloids in Coptidis Rhizoma from Different Areas

关键词:  黄连  生物碱  道地药材  高效液相色谱法

Key words:  Coptidis Rhizoma  alkaloid  Genuine medicinal  HPLC

摘要:

目的 测定不同产地黄连药材加工品中盐酸药根碱、盐酸非洲防己碱、盐酸表小檗碱、盐酸黄连碱、盐酸巴马汀、盐酸小檗碱的含量，为黄连药材的真伪鉴别提供依据。方法 色谱条件为Phenomenex Gemini-NX C₁₈色谱柱 (250 mm×4.6 mm，5 μm)；流动相为乙腈-30 mmol?L^-1碳酸氢铵溶液(每1 000 mL碳酸氢铵溶液含7 mL 氨水，1 mL三乙胺)梯度洗脱；流速1.0 mL·min^-1；检测波长270 nm；柱温30 ℃。结果 黄连药材中6个生物碱含量均较高，且其中盐酸药根碱、盐酸防己碱、盐酸表小檗碱、盐酸黄连碱、盐酸巴马汀和盐酸小檗碱的含量分别不低于0.30%，0.40%，1.10%，2.02%，1.45%，6.58%。结论 方法准确、可行，可作为黄连药材质量的定量依据。

Abstract:

OBJECTIVE To determine the contents of jateorrhizine hydrochloride, columbamine hydrochloride, epiberberine hydrochloride, coptisine hydrochloride, palmatine hydrochloride, and berberine hydrochloride in crude Coptidis Rhizoma and explore the quality relationship of Coptidis Rhizoma, which providing a basis for distinguishing. METHODS The samples were separated by a Phenomenex Gemini-NX C₁₈ column (250 mm×4.6 mm, 5 μm) using acetonitrile-30 mmol·L^-1 ammonium bicarbonate (each 1 000 mL ammonium bicarbonate contained 7 mL ammonia water and 1 mL triethylamine) gradient system as mobile phase at the flow rate of 1.0 mL·min^-1, the detective wavelength was 270 nm and the column temperature was 30 ℃. RESULTS The quality of Coptidis Rhizoma from different areas is high, the lowest average contents of jateorrhizine hydrochloride, columbamine hydrochloride, epiberberine hydrochloride, coptisine hydrochloride, palmatine hydrochloride, and berberine hydrochloride in Coptidis Rhizoma are 0.30%, 0.40%, 1.10%, 2.02%, 1.45%, 6.58%. CONCLUSION This method is rapid, accurate, sensitive, reproducible. It is proved to be suitable for the quality control of Coptidis Rhizoma.