减压特号对慢性应激大鼠的抗抑郁作用及机制研究

Antidepressant Effects of Jianyatehao(JYTH) and Its Possible Mechanisms in Chronically Stressed Rats

关键词:  减压特号  慢性应激  皮质酮  白介素-1β  白介素-6  单胺递质

Key words:  Jianyatehao  chronically stressed  corticosterone  IL-1β  IL-6  monoamines

摘要:

目的 探讨中药复方减压特号(Jianyatehao，JYTH)对慢性应激大鼠抑郁样行为的改善作用及其对肾上腺结构功能、免疫系统和单胺递质系统的影响。方法 大鼠随机分为正常对照组、应激模型组、JYTH(低、中、高)剂量组及丙咪嗪组。建立大鼠慢性应激模型，测定应激大鼠的开野行为和糖水消耗量。ELISA法测定大鼠血清皮质酮、IL-1β和IL-6的水平，HPLC测定应激大鼠不同脑区的单胺递质水平。结果 与慢性应激组相比，JYTH低、中、高剂量组明显增加大鼠在开野箱中的活动性，并增加其对糖水的偏爱性，明显降低血清皮质酮、IL-1β和IL-6的水平，明显增加不同脑区单胺递质含量。结论 JYTH可以改善慢性应激引起的抑郁样行为，其作用机制可能与降低血清皮质酮水平、保护肾上腺结构完整、增强免疫功能和提高脑内单胺递质含量有关。

Abstract:

OBJECTIVE To investigate the antidepressant effects of Jianyatehao(JYTH), a traditional Chinese medicine formula, and its possible mechanisms. METHODS SD rats were randomly divided into 6 groups: normal group, model group, and four treated groups treatment with high dose JYTH(70 g·kg^-1), moderate dose JYTH(35 g·kg^-1) and low dose JYTH (17.5 g·kg^-1) and imipramine group(10 mg·kg^-1), respectively. The chronically stressed model was established, and the activities in the open-field box and sucrose consumption stress rats were observed respectively. Determine the levels of corticosterone, IL-1β and IL-6 in stressed rats using ELISA and measure the monoamines in various brain regions by Sucrose. Furthermore, the contents of 5-HT, noradrenaline, dopamine and 5-HIAA in various brain areas were measured using HPLC with electrochemical detection with minor modifications. RESULTS Compared with the chronically stressed rats, JYTH(17.5, 35, 70 g·kg^-1) significantly reversed the depressive-like behaviors of chronically stressed rats, which can increase the reactiveness in open-field box and the preference of treatments improved the chronically stressed-induced increase of serum corticosterone, IL-1β and IL-6 levels. JYTH (17.5, 35, 70 g·kg^-1) treatments also increased the monoamines in various brain regions. CONCLUSION JYTH has significant antidepressant effects, which is probably related to the reduce of the levels of serum corticosterone and the protection of the structural integrity of the adrenal and the improvement of the immunity functions and the increase of the monoamines in brain.