栾华挥发油的提取及GC-MS分析

Extraction and GC-MS Analysis of Essential Oil from Flower of Paniculate Goldraintree

关键词:  栾华  挥发油  水蒸气蒸馏法  气相色谱-质谱联用法

Key words:  flower of paniculate goldraintree  essential oil  steam distillation  GC-MS

摘要:

目的 分析栾华的挥发油成分。方法 水蒸气蒸馏法提取栾华挥发油，并用GC-MS对其成分进行分析，并用面积归一化法测定各组分的相对含量。其中浸泡时间为20 h，加热8 h至挥发油量不再增加为止；GS-MS条件：DB-5MS毛细管色谱柱(0.2 mm×30 m，0.25 μm)；载气为高纯度氦气，流速为1 mL·min^-1；质谱分析条件：EI电离源70 eV；离子源温度200 ℃。结果 鉴定出了35个组分占总出峰面积的98.8%，并确定了其中主要成分的相对含量。结论 栾华挥发油的主要成分为：3-甲基-4-羰基戊酸(49.71%)、n-十六酸(16.16%)、肉豆蔻酸(2.62%)、亚麻酸(2.38%)、9,12-十八碳二烯酸(2.36%)、2-十五酮(2.32%)、正三十六烷(2.17%)、苯乙醛(2.05%)。

Abstract:

OBJECTIVE To analyze the compositions of essential oil from flower of paniculate goldraintree. METHODS To isolate by steam distillation and analyze by GC-MS. Relative content of each component was determined by area normalization. Immersion them 20 hours and heating them to 8 hours until the essential oil did not increase. GS-MS conditions: DB-5MS capillary column (0.2 mm×30 m, 0.25 μm), carrier gas was high purity helium, flow rate was 1 mL·min^-1. MS conditions: EI ionization 70 eV, ion source temperature was 200 ℃. RESULTS Thirty-five compounds, representing 98.8% of total oil, were indentified and their relative amount was determined. CONCLUSION The oil is rich in 3-methyl-4-oxopentanoic acid (49.71%), n-hexadecanoic acid (16.16%), myristic acid(2.62%), linolenic acid(2.38%), (Z,Z)-9,12-octadecadienoic acid(2.36%), 2-pentadecanone(2.32%), hexatriacontane(2.17%), benzeneacetaldehyde(2.05%).