秦艽地上部分极性成分的SFE-GC-MS分析

Chemical Constituents Analysis in Ground Part of Gentiana Macrophylla Pall. by SFE-GC-MS

关键词:  秦艽  地上部分  超临界二氧化碳流体萃取法  气相色谱-质谱联用法

Key words:  Gentiana macrophylla Pall.  ground part  SFE-CO2  GC-MS

摘要:

目的 对秦艽地上部分超临界萃取提取物中极性成分进行分析。方法 采用超临界二氧化碳流体萃取法(SFE-CO₂)对秦艽的花及茎叶进行提取，利用气相色谱-质谱联用法(GC-MS)对提取物中的极性成分进行分离分析，并计算各成分的相对百分含量。色谱柱：AT-SE54毛细管柱(30 m×0.25 mm×0.25 mm)；进样口温度：260 ℃；程序升温：初始80 ℃，以4 ℃·min^-1升至290 ℃，保持30 min；载气为高纯氦气，流速1.2 mL·min^-1；离子源温度：230 ℃；离子源电离能：70 eV。结果 从秦艽花中共分离出72个化合物，鉴定出其中70个，占提取物的84.1%；从秦艽茎叶中共分离出67个化合物，鉴定出其中66个，占提取物的93.1%。结论 该实验结果为了解秦艽地上部分的化学成分及进一步开发利用提供了依据。

Abstract:

OBJECTIVE To identify compounds in supercritical fluids extraction of ground part of Gentiana macrophylla Pall.. METHODS GC-MS was used to analyse the constituents and the sample was extracted by supercritical fluids. The AT-SE54 capillary column(30 m×0.25 mm×0.25 μm)was used and inlet temperature was maintained at 260 ℃. The column oven was begun at 80 ℃, then programmed from 80 ℃ to 290 ℃ at 4 ℃·min^-1, and hold for 30 min. Helium at a constant flow rate of 1.2 mL·min^-1 was used as carrier gas. Ion source temperature was 230 ℃ and ionization energy was 70 eV. RESULTS A total of 72 components were separated and 70 components were identified from flowers of Gentiana macrophylla Pall., accounting for 84.1% of total extractant. A total of 67 components were separated and 66 components were identified from stems and leaves of Gentiana macrophylla Pall., accounting for 93.1% of total extractant. CONCLUSION The results will provide important foundation for understanding the constituents and further exploitation for ground part of Gentiana macrophylla Pall..