卷烟烟气中木犀草素和黄芩苷混合物对呼吸系统作用的研究

Experimental Study of Luteolin and Baicalin Mixture in Cigarette Smoke on Respiratory System

关键词:  特制卷烟  卷烟烟气  黄芩苷  木犀草素  呼吸系统

Key words:  purpose-made cigarette  cigarette smoke  baicalin  luteolin  respiratory system

摘要:

目的 研究卷烟烟气中木犀草素和黄芩苷对呼吸系统的作用。方法 将木犀草素和黄芩苷混合物加入卷烟制成特制卷烟，以动物吸烟机使混合物通过烟气接触动物呼吸系统。采用小鼠氨水引咳法制作咳嗽模型；以小鼠酚红排泄法评价气道分泌液量；以豚鼠整体动物药物引喘法评价对支气管痉挛的作用；并进行木犀草素和黄芩苷混合物经消化道给药和特制卷烟的急性毒性试验。结果 与对照卷烟比较，特制卷烟可明显减少咳嗽，增加气道分泌液量，减轻动物呼吸道刺激。结论 特制卷烟中木犀草素和黄芩苷能降低卷烟的呼吸系统危害性。

Abstract:

OBJECTIVE To study the effect of luteolin and baicalin mixture in purpose-made cigarette smoke on respiratory system. METHODS Purpose-made cigarette was made by adding luteolin and baicalin mixture into cigarette. Mixture in smoke contacted with respiratory system by smoking machine. The cough model induced by ammonia in mice was used. The effect of mixture on tracheal excretion was evaluated by phenol red excretion test in mice. The action of luteolin and baicalin mixture on asthma was investigated in histamine and acetylcholine induced-asthma guinea pig. Acute toxicity tests of oral luteolin and baicalin mixture and purpose-made cigarette were conducted. RESULTS Luteolin and baicalin mixture in smoke reduced the frequency of cough ammonia-induced in mouse, increased the tracheal excretion compared with control cigarette, and eased the incitement to respiratory system. CONCLUSION Luteolin and baicalin mixture in purpose-made cigarette smoke can diminish perniciousness of cigarette and improve the quality of cigarette in sensory evaluation to some extent.