| 引用本文: | 罗宏丽，肖顺林，冯碧敏.HPLC测定复方地米水杨酸酊中硝酸咪康唑的含量[J].中国现代应用药学,2011,28(8):765-767. |
| LUO Hongli, XIAO Shunlin, FENG Bimin.Determination of Miconazole Nitrate in Compound Dexamethasone Acetate Salicylate Tincture by HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2011,28(8):765-767. |
|
| 摘要: |
| 目的 建立高效液相色谱法测定复方地米水杨酸酊中硝酸咪康唑的含量。方法 色谱柱为Hypersil C18柱(250 mm×4.6 mm，5 mm)，流动相为乙腈-甲醇-0.5%醋酸铵溶液(40∶40∶15)，流速为1.0 mL·min-1，检测波长为220 nm，柱温为35℃。结果 硝酸咪康唑在30.72~81.92 μg·mL-1内与峰面积呈良好的线性关系(r=0.999 7)；平均回收率为99.6%，RSD=0.68%(n=9)。结论 本方法简便快速、准确、重复性好，可用于该制剂中硝酸咪康唑的含量测定。 |
| 关键词: 硝酸咪康唑 复方地米水杨酸酊 高效液相色谱法 含量测定 |
| DOI： |
| 分类号: |
| 基金项目: |
|
| Determination of Miconazole Nitrate in Compound Dexamethasone Acetate Salicylate Tincture by HPLC |
|
LUO Hongli, XIAO Shunlin, FENG Bimin
|
|
Department of Pharmacy, the Affiliated Hospital of Luzhou Medical College, Luzhou 646000, China
|
| Abstract: |
| OBJECTIVE To establish an HPLC method to determine miconazole nitrate in compound dexamethasone acetate salicylate tincture. METHODS The analysis was carried out at 35 ℃ on a Hypersil C18 column (250 mm×4.6 mm, 5 mm). The mobile phase consisted of acetonitrile-methanol-0.5% ammonium acetate solution (40∶40∶15) with the flow rate of 1.0 mL·min-1. The detection wavelength was 220 nm. The column temperature was 35 ℃. RESULTS The calibration curve of miconazole nitrate was linear over the range of 30.72-81.92 μg·mL-1(r=0.999 7). The average recovery was 99.6% (RSD=0.68%, n=9). CONCLUSION This method is simple, accurate, reproducible and applicable for the content determination of miconazole nitrate in compound dexamethasone acetate salicylate tincture. |
| Key words: miconazole nitrate compound dexamethasone acetate salicylate tincture HPLC content determination |
