| 引用本文: | 战峰，李超，韩泳平.回归正交设计优化西红花苷的提取工艺[J].中国现代应用药学,2011,28(8):729-732. |
| ZHAN Feng, LI Chao, HAN Yongping.Optimization of Extraction of Crocin from Crocus Sativus L. by Orthogonal Regression Design[J].Chin J Mod Appl Pharm(中国现代应用药学),2011,28(8):729-732. |
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| 摘要: |
| 目的 研究从西红花中提取西红花苷的最佳工艺条件。方法 以西红花苷的提取率为指标，在使用单因素试验方法考察乙醇浓度、提取时间、提取温度对西红花中西红花苷提取率的基础上，应用回归正交方法建立3元2次方程优化西红花苷的提取工艺。结果 最佳的提取工艺是：乙醇浓度52%，提取时间30 min，提取温度30 ℃。此条件下，提取率为0.643 5 mg·g-1。结论 在优化的提取工艺下，西红花中西红花苷提取率较高。 |
| 关键词: 西红花 西红花苷 回归正交试验 |
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| 基金项目:国家外专局项目(Y-2010-12) |
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| Optimization of Extraction of Crocin from Crocus Sativus L. by Orthogonal Regression Design |
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ZHAN Feng, LI Chao, HAN Yongping
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Southwest University for Nationalities, College of Chemistry and Environment Protection Engineering, Chengdu 610041, China
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| Abstract: |
| OBJECTIVE To study the optimal extraction condition of crocin from Crocus sativus L. METHODS Taking the total extraction ratio of crocin from Crocus sativus L. as an index, the quadratic orthogonal regression design which was based on the single factor method including the concentration of ethanol, extraction time and extraction temperature was employed to build the equation and to optimize the extraction technique. RESULTS The optimized condition was as follows: the concentration of ethanol was 0.52%, extracted 30 minutes at the temperature of 30 ℃, and the extraction ratio reached 0.643 5 mg·g-1. CONCLUSION Under the optimal extraction condition, the total extraction ratio of crocin from Crocus sativus L. is much higher. |
| Key words: Crocus sativus L. crocin orthogonal regression design |
