RP-HPLC测定杜仲叶活性成分的含量

Determination of Active Constitutents in Folium Eucommiae by RP-HPLC

关键词:  反相高效液相色谱法  杜仲叶  含量测定  绿原酸  京尼平苷

Key words:  RP-HPLC  folium eucommiae  content determination  chlorogenic acid  genipoide

摘要:

目的建立同时测定杜仲叶中活性成分绿原酸、京尼平苷含量的反相高效液相色谱法。方法采用YMC-pack ODS-A色谱柱（250 mm×4.6 mm，5 μm），以甲醇-水-冰醋酸（20∶80∶1.5）为流动相，检测波长237 nm，柱温30 ℃，流速1.0 mL·min-1。结果绿原酸在0.041 6～0.624 0 μg内，有较好的线性关系(r=0.999 2)，绿原酸的加样回收率为102.92%，RSD为1.49%；京尼平苷在0.081 6～1.224 0 μg内，有较好的线性关系(r=0.998 9)，京尼平苷的加样回收率为102.35%，RSD为1.20%。杜仲叶中绿原酸含量为2.801 mg·g-1、京尼平苷含量为0.273 mg·g-1。结论所建立的方法简便、准确、可靠，可作为杜仲叶的质量控制方法。

Abstract:

OBJECTIVE To establish a new reversed phase high-performance liquid chromatography (RP-HPLC) method for the determination of chlorogenic acid and genipoide, the active constitutents in folium eucommiae simultaneously. METHODS YMC-pack ODS-A column (250 mm×4. 6 mm, 5 μm) was used and the mobile phase was methanol-water-acetic acid (20∶80∶1.5) at the flow rate of 1.0 mL·min-1. The detection wavelength was 237 nm and the temperature of column was 30 ℃. RESULTS The linear range of chlorogenic acid and genipoide were 0.041 6-0.624 0 μg(r=0.999 2) and 0.081 6-1.224 0 μg(r=0.998 9). The average recoveries were 102.92% and 102.35%,respectively. The RSD were 1.49% and 1.20%. The content of chlorogenic acid and genipoide in folium eucommiae were 2.801 mg·g-1 and 0.273 mg·g-1. CONCLUSION The method is simple, accurate and reliability, with good repeatability, and can be used for the quality control of folium eucommiae.