| 引用本文: | 李钦1,郑成2,李晓誉1,张信岳1*.HPLC测定脂清颗粒中2，3，5，4′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷的含量[J].中国现代应用药学,2009,(2):142-144. |
| LI Qin1,ZHENG Cheng2,LI Xiaoyu1,ZHANG Xinyue1*.Determination of 2，3，5，4′-tetrahydroxystilbene-2-O-β-D-glucoside Content in Zhiqing Granule by HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2009,(2):142-144. |
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| 摘要: |
| 目的建立脂清颗粒中2，3，5，4′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷的高效液相色谱测定方法。方法采用Dikma Technologies Diamonsil C18柱( 250 mm×4.6 mm，5μm)；乙腈-水（25∶75）为流动相；流速1.0 mL·min-1；检测波长为320 nm。 结果2，3，5，4′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷平均回收率为101.5%，RSD为1.0%（n=9）。 结论本法简便、快速、灵敏，重复性好，可用于脂清颗粒的质量控制。 |
| 关键词: 高效液相色谱法 脂清颗粒 2，3，5，4′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷 |
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| Determination of 2，3，5，4′-tetrahydroxystilbene-2-O-β-D-glucoside Content in Zhiqing Granule by HPLC |
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LI Qin1,ZHENG Cheng2,LI Xiaoyu1,ZHANG Xinyue1*
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| Abstract: |
| OBJECTIVE To eatablish an HPLC for the determnination of 2，3，5，4′-tetrahydroxystilbene-2-O-β-D-glucoside content in Zhiqing granule.METHODS Using Dikma Technologies Diamonsil C18 column (250 mm×4.6 mm, 5 μm), the mobile phase was acetonitrile-water (25∶75)at the flow rate of 1.0 mL·min-1.The UV detection wavelength was 320 nm. RESULTS The average recovery was 101.5%, RSD was 1.0% (n=9). CONCLUSION The method is simple and can be used for the quality control of Zhiqing granule. |
| Key words: HPLC Zhiqing granule 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside |
