| 引用本文: | 梁泽华1，尹丽娜2，杨燕1，熊耀康1*.不同炮制方法对川乌双酯型生物碱含量的影响[J].中国现代应用药学,2009,(4):288-291. |
| LIANG Zehua1,YIN Lina2,YANG yan1,XIONG Yaokang1*.The Effect of the Processing Methods on the Content of Diacrylate Alkaloids of Radix Aconiti[J].Chin J Mod Appl Pharm(中国现代应用药学),2009,(4):288-291. |
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| 摘要: |
| 目的研究炮制方法对川乌双酯型生物碱含量的影响。方法将生川乌分别通过药典法、黑豆法和米醋法进行炮制，以RP-HPLC结合梯度洗脱的色谱方法为检测手段，比较分析不同川乌炮制方法对双酯型生物碱（主要为乌头碱、次乌头碱）含量的影响。结果3种炮制方法均可显著降低生川乌中乌头碱、次乌头碱的含量。所建立的色谱方法可定量分析炮制品中双酯型生物碱。结论不同炮制方法对生川乌均能起到减毒作用。 |
| 关键词: 川乌 炮制方法 双酯型生物碱 高效液相色谱法 梯度洗脱 |
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| The Effect of the Processing Methods on the Content of Diacrylate Alkaloids of Radix Aconiti |
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LIANG Zehua1,YIN Lina2,YANG yan1,XIONG Yaokang1*
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| Abstract: |
| OBJECTIVE To study the effect of the processing methods on the content of diacrylate alkaloids of Radix Aconiti (RA). METHODS The RA was processed,using Ch.P,Black Bean and Vinegar law metheds.RP-HPLC with gradient elution was established for determining the content of diacrylate alkaloids(mainly aconitine、hypaconitine).RESULTS Three processing methods can significantly reduce the contents of aconitine and hypaconitine. Chromatographic methods established can be applied to the quantitative analysis of the diacrylate alkaloids of processed products.CONCLUSION The described processing methods can reduce the content of diacrylate alkaloids of RA. |
| Key words: Radix Aconiti processing methods diacrylate alkaloids HPLC gradient elution |
