五味子醇提部位对反复脑缺血再灌注致记忆障碍模型小鼠学习记忆的影响

Effect of Schisandra Chinensis Baill Distilled by Ethanol on the Ability of Learning and Memory in Memory Impairment Mice Model Induced by Repeated Cerebral Ischemia-reperfusion

关键词:  五味子醇提部位  反复脑缺血再灌  记忆障碍  学习记忆

Key words:  Schisandra Chinensis Baill distilled by ethanol  repeated cerebral ischemia-reperfusion  memory impairment  learning and memory

摘要:

目的 探讨五味子醇提部位对反复脑缺血再灌注致记忆障碍模型小鼠学习记忆的影响。方法 将动物随机分成6组，分别灌服尼莫地平片混悬液、银杏叶混悬液及大、小剂量五味子醇提部位混悬液，假手术组（空白组）和模型组给予同体积生理盐水，1次·d-1，连续给药15 d，采用双侧颈总动脉夹闭,建立反复脑缺血再灌注致小鼠学习记忆障碍模型。术后48 h进行行为学检测。跳台法：将小鼠放入跳台仪的小盒内进行训练，24 h后重新测试小鼠记忆情况，观察并记录小鼠首次从跳台上跳下的潜伏期及5 min内错误次数；避暗法：将小鼠尾部对着进入暗室的小口放入测定盒中，进行训练，24 h后重新测定小鼠记忆情况，观察并记录小鼠首次进入暗室的潜伏期及5 min内进入暗室遭电击的次数。结果 跳台法：大剂量五味子醇提部位组够显著延长造模后小鼠触电潜伏期，明显减少测试期小鼠跳台错误反应次数；避暗法：大剂量五味子醇提部位组明显延长造模后小鼠潜伏期，明显减少测试期小鼠错误反应次数。结论 五味子醇提部位对反复脑缺血再灌致记忆障碍模型小鼠学习记忆具有一定改善作用。

Abstract:

OBJECTIVE To observe the effect of Schisandra Chinensis Baill distilled by ethanol on the ability of learning and memory in memory impairment mice model induced by repeated cerebral ischdmia-reperfusion. METHOD The mice were randomly divided into six groups,respectively irrigating the groups with Nimodipine suspension,ginkgo leaf tablet suspension, large and small doses of Schisandra Chinensis Baill distilled by ethanol, sham group (blank and the model group for the same volume of saline, Once a day,15days for delivery, Mice model of cerebral ischemia-reperfuqion injury was established by bilateral common carotid artery blocking.48 hours after operation, begin the experiment of behavior. Step down test: the mice were put into the apparatus of step down for discipline and the training, 24 hours later, retest the memory of the mice, register the delitescence of the set up and the times of the mistakes in 5 minutes. Evading dark test: the mice were put into the darkroom, turn on the electrical source for discipline and the training, 24hours later, retest the memory of the mice .Register the delitescence of enter into the darkroom and the tip-and-run times. RESULTS Step down test: the large doses of Schisandra Chinensis Baill distilled by ethanol could notably prolong the latent phase of getting an electric shock，and could obviously decrease the error times in Step down tests; Evading dark test: the large doses of Schisandra Chinensis Baill distilled by ethanol could markedly prolong the latent phase for model mice in learning tests, and could notably decrease the error times in learning tests. CONCLUSION Schisandra Chinensis Baill distilled by ethanol could significantly improve on the ability of learning and memory of memory impairment mice model induced by repeated cerebral ischdmia-reperfusion.