不同采收期紫丁香叶中原儿茶酸、原儿茶醛和酪醇的含量测定

Determination of Protocatechuic Acid, Protocatechuic Aldehyde and Tyrosol in Different Seasons Collections of Syringa Oblate Leaves

关键词:  紫丁香  采收期  原儿茶酸  原儿茶醛  酪醇

Key words:  Syringa oblate Lindl.  collection periods  protocatechuic acid  protocatechuic aldehyde  tyrosol

摘要:

目的测定不同采收期紫丁香叶中原儿茶酸、原儿茶醛、酪醇的含量。方法采用RP-HPLC直接测定。YWG-C18色谱柱(4.6 mm×250 mm，10 μm)；流动相为甲醇-0.125%冰醋酸溶液(10︰90)，流速1.0 mL·min-1；检测波长275 nm。结果原儿茶酸在0.08～0.56 μg内呈良好线性，回归方程为Y = 83 995 X－10 368, r = 0.999 5；原儿茶醛在0.01～0.07 μg内呈良好线性，回归方程为Y = 111 202 X－35 323, r =0.999 8；酪醇在0.42～2.94 μg内呈良好线性，回归方程为Y = 139 922 X－32 003, r = 0.999 6。紫丁香叶中原儿茶酸的含量10月份最高，原儿茶醛和酪醇的含量4月份最高。结论该方法简便、快速、准确可靠，适用于紫丁香叶中原儿茶酸、原儿茶醛、酪醇多种成分的同时测定；适用于紫丁香叶的质量控制。结果表明紫丁香叶以10月份或4～5月份采收为宜。

Abstract:

OBJECTIVE To establish a RP-HPLC method to determine the contents of protocatechuic acid, protocatechuic aldehyde and tyrosol in Syringa oblate. METHODS The samples were determined by RP-HPLC on a YWG C18 column(4.6 mm×250 mm，10 μm), using methanol-0.125% glacial acetic acid solution(10∶90)as the mobile phase with flow rate of 1 mL·min-1and detected by UV at 275 nm. RESULTS The regression equation of protocatechuic acid: Y=83 995 X－10 368, r=0.999 5，the linear ranges was at 0.08－0.56 μg. The regression equation of protocatechuic aldehyde: Y=111 202 X－35 323, r=0.999 8, the linear ranges was at 0.01－0.07 μg. The regression equation of tyrosol: Y=139 922 X－32 003, r=0.999 6, the linear ranges was at 0.42～2.94 μg. The samples collected in October had the highest contents of protocatechuic acid, while the highest content of protocatechuic aldehyde and tyrosol was in April. CONCLUSION The method is simple，rapid and accurate, and can determine the three components at one time. It can be used for quality evaluation of Syringa oblate. The results show that the optimum collection time of Syringa oblate can be in October or April to May.