HPLC荧光法检测转铁蛋白修饰的阿霉素脂质体在小鼠体内的含量

Determination of Doxorubicin Liposomes Modified with Transferrin in PCR Mice by HPLC With Fluorescence Detector

关键词:  转铁蛋白  阿霉素脂质体  反相高效液相色谱

Key words:  transferring  doxorubicin liposomes  RP-HPLC

摘要:

目的 建立转铁蛋白修饰的阿霉素脂质体在小鼠体内组织浓度的测定方法。方法 选择乙腈-5 mmol·L-1乙酸铵水溶液(30∶70)为流动相；色谱柱：DIKMA Diamonsil C18柱 (4.6 mm×150 mm，5 μm)；流速1.0 mL·min-1；检测波长：激发波长为480 nm，发射波长为550 nm；柱温：30 ℃。结果 本实验所用方法线性关系良好，且具有专一性强、准确性、灵敏度高的优点，符合生物样本分析的要求。结论 可用本法快速、简便、准确地测定小鼠组织样品中转铁蛋白修饰的阿霉素脂质体的含量。

Abstract:

OBJECTIVE To establish a sensitive and specific method for the determination of doxorubicin liposomes modified with transferrin in PCR mice by HPLC with fluorescence detector. METHODS RP-HPLC with fluorescence detector was used. ADIKMA Diamonsil C18 column (4.6 mm×150 mm，5 μm) was used with the mobile phase of acetonitrile-5 mmol·L-1 ammonium acetate (30∶70). The detection wavelength was EX 480 nm，EM 550 nm and the temperature was at 30 ℃. The flow velocity was 1.0 mL·min-1. RESULTS The calibration curve showed a good linearity. The result of the extraction recovery, the intra-day and inter-day RSD of doxorubicin were all analytical for the determination. CONCLUSION The method is sensitive, fast, precise and reliable to operate, and is suitable for the analysis of doxorubicin liposomes modified with transferrin in PCR mice.