鬼箭锦鸡儿中紫檀烷类化合物抗真菌活性研究

Antifungal Activity of Pterocarpans from Caragana jubata (pall.) Poir

关键词:  鬼箭锦鸡儿  紫檀烷  抗真菌活性

Key words:  Caragana jubata (pall.) Poir.  Pterocarpans  antifungal activity

摘要:

目的研究鬼箭锦鸡儿中紫檀烷类化合物的抗真菌活性。方法在生物活性指导下,利用聚酰胺、硅胶常规色谱和制备型高效液相色谱方法进行化合物分离，采用波谱技术和化学方法鉴定化合物的结构。最低抑菌浓度(MIC)测定参照美国国家临床实验室标准化委员会(NCCLS)推荐的M27-A方案中的微量肉汤稀释法。结果鬼箭锦鸡儿整株植物氯仿部分显示好的抗真菌活性，活性追踪分离得到五个紫檀烷类化合物，分别是3-甲氧基高丽槐素(1)、高丽槐素(2)、3-甲氧基-9羟基紫檀烷(3)、3，9-二甲氧基紫檀烷(4)和3-甲氧基-4，9二羟基紫檀烷(5)，其中化合物2面对三种念珠菌菌株，显示出潜在的抗真菌活性，最低抑菌浓度范围为6.25～25 μg·mL-1。结论所有化合物均为首次从该植物分离得到,并且生物活性结果确认紫檀素类化合物是有效的抗真菌成分。

Abstract:

OBJECTIVE To study the antifungal activity of pterocarpans from Caragana jubata (pall.) poir.. METHODS The compounds have been separated and purified by polyamine resin and silica gel column chromatography as well as the preparative HPLC method from the active fraction. The minimum inhibitory concentration(MIC) were measured by broth microdilution method according to document M27-A published by the National Committee for Clinical Laboratory Standards (NCCLS). RESULTS The CHCl3 extract of the MeOH extract prepared from the whole plant of Caragana jubata (pall.) Poir．Exhibited good antifungal activity. Bioassay-directed fractionation led to the purification of five pterocarpans, 3-methoxy maackiain (1), maackiain (2), 3-methoxy-9-hydroxy pterocarpan (3), 3,9-dimethoxy pterocarpan (4) and 3-methoxy-4,9-dihydroxy pterocarpan (5), in which compounds 2 showed potential antifungal activity against three Candida strains at the level of MICs 6.25-25 μg·mL-1. CONCLUSION All of the compounds were isolated from the title plant for the first time. And biological validation showed pterocarpans were responsible for antifungal activity.